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首页> 外文期刊>African Journal of Biotechnology >Comparative genomic in situ hybridization analysis on the chromosomes of five grass species with rice genomic DNA probe
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Comparative genomic in situ hybridization analysis on the chromosomes of five grass species with rice genomic DNA probe

机译：水稻基因组DNA探针对5种草种染色体的比较基因组原位杂交分析

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Comparative genomic?in situ?hybridization (cGISH) with biotin-labeled rice genomic DNA to the chromosomes of?Zea mays,?Hordeum vulgare,?Sorghum bicolor, Setaria italic?and?Secale cereale?were conducted to analyze genomic homology between rice and other grass (Gramineae) speices. At 75% stringency, the rice DNA probe generated large numbers of signals dispersedly distributed over all chromosomes in all target species. The nucleolar organizing regions (NORs), a few telomeres, most centromeric regions and numerous interstitial sites were detected. The signals in small genomes were relatively sparse and unevenly distributed along chromosomes, whereas those in large genomes were dense and basically evenly distributed. Chromosomal?in situ?suppression (CISS) experiments indicated that the signals represented the hybridization of repetitive DNAs?is?common between rice and the target genome. cGISH conducted at low and high stringency revealed that repetitive DNAs with?～65% homology are rich and those with?～90% homology are rare between rice and maize or barley. cGISH using maize genomic DNA probe to the rice chromosomes generated relatively sparse and unevenly distributed signals. Our results reveal that there exist besides rDNA and telomeric repeats, some other conserved repetitive DNAs among grass species of different subfamilies. Our results also suggest that ancient conserved repetitive DNAs had undergone considerable amplification in large grass genomes during evolution.
机译：用生物素标记的水稻基因组DNA与玉米，玉米，大麦，双色高粱，斜纹狗尾草和谷类谷物的染色体进行比较基因组原位杂交（cGISH），以分析水稻与水稻之间的基因组同源性其他草（Gramineae）。严格度为75％时，水稻DNA探针会产生大量分散在所有目标物种的所有染色体上的信号。核仁组织区（NORs），少数端粒，多数着丝粒区域和大量的间隙位置。小基因组中的信号相对稀疏，沿着染色体分布不均匀，而大基因组中的信号则密集且基本上均匀分布。染色体原位抑制（CISS）实验表明，该信号代表水稻和目标基因组之间常见的重复DNA杂交。在高和低严格条件下进行的cGISH分析表明，在水稻与玉米或大麦之间，具有？〜65％同源性的重复DNA丰富，而具有？〜90％同源性的重复DNA很少。 cGISH使用玉米基因组DNA探针对水稻染色体产生相对稀疏且分布不均的信号。我们的研究结果表明，除了rDNA和端粒重复序列外，不同亚科的草种中还存在其他一些保守的重复DNA。我们的结果还表明，古代保守的重复DNA在进化过程中已在大型草基因组中经历了可观的扩增。

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