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Application of Trichoderma harziunum T22 as a biofertilizer supporting maize growth

机译:哈氏木霉T22作为支持玉米生长的生物肥料的应用

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This study was conducted to investigate the effect of?Trichoderma harzianum?strain (T22) on the growth and development of maize (Zea mays) plants.?T. harzianum?was applied to the grains in two different treatments. The first one was performed by inoculating the soil with the air dried growth of the tested fungus in three different levels (2, 4 and 6 g kg-1) before sowing. The second treatment was conducted by treating the seeds with different concentrations of metabolic solution of?T. harzianum(100, 200 and 300?μl) before sowing time. We compared?these two types of treatments to determine which one has more benefit for improving the growth of maize plants.?Results obtained revealed that the application of?T. harzianum?caused increases in all measured parameters which include growth parameters, chlorophyll content, starch content, nucleic acids content, total protein content and phytohormones content of maize plants, when applied to the soil or the seeds but the magnitude of these increases was much more pronounced in case of plants developed from seeds treated with various concentrations of metabolic solution of?T. harzianum. The?sodium dodecyl sulphate-polyacryalmide gel electrophresis (SDS-PAGE)?electrophoretic pattern of proteins extracted from maize leaves revealed the appearance of newly protein bands. It was concluded that the uses of?T. harzianumimproves the growth and development of maize plants.
机译:本研究旨在研究哈茨木霉菌株(T22)对玉米(Zea mays)植物生长和发育的影响。在两种不同的处理方法中,将harzianum?应用于谷物。第一次播种是通过在播种前用三种不同水平(2、4和6 g kg-1)的测试真菌风干生长接种土壤。通过用不同浓度的ΔT代谢溶液处理种子进行第二种处理。播种前,先采哈茨草(100、200和300?l)。我们比较了这两种类型的处理方法,以确定哪一种对改善玉米植物的生长更有益处。如果将哈茨木霉菌施用到土壤或种子上,则所有测量参数的增加,包括生长参数,叶绿素含量,淀粉含量,核酸含量,总蛋白质含量和植物激素含量,但这些增加的幅度更大如果植物是用各种浓度的ΔT代谢溶液处理过的种子发育而来的,则是明显的。哈尔扎努姆。从玉米叶片提取的蛋白质的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)电泳图谱显示出新的蛋白质带。得出结论,ΔT的用途。 harzianum改善了玉米植物的生长发育。

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