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首页> 外文期刊>African Journal of Biochemistry Research >Protease inhibition in cowpea pod-sucking bug species (Hemiptera: Heteroptera) using cereal and legume grain extracts
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Protease inhibition in cowpea pod-sucking bug species (Hemiptera: Heteroptera) using cereal and legume grain extracts

机译：使用谷类和豆类谷物提取物抑制cow豆荚bug虫（半翅目：异翅目）中的蛋白酶

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Selected grain extracts were assayed against digestive proteases of Anoplocnemis curvipes, Clavigralla tomentosicollis and Mirperus jaculus with a view to identifying suitable sources of protease inhibitors against the cowpea pests. Laboratory bioassays showed that grains of a local soybean variety Kìshí and a cowpea landrace NG/SA/07/0098 contain potent inhibitors although the former produced a consistently higher level of inhibition in each of the three pests. Protease activity was highest in C. tomentosicollis (1.06 μmol/mg) followed by M. jaculus (0.94 μmol/mg) and A. curvipes (0.69 μmol/mg) but inhibition was highest in M. jaculus. The optimum temperature range for the inhibitors was 40-90°C while optimum inhibitory activity occurred in weak acidic to neutral media, pH 4-7. There was total protease inhibition in the pests when soybean and cowpea extracts were combined against gut extracts in soybean-biased volumetric ratios of 0.7:0.3 and 0.8:0.2. This synergistic efficacy was superior to those of two commercial products. The inhibitors were stable for 10-50 min at 80°C but got denatured within few minutes of exposure to higher temperatures. Obtained results showed that the soybean and cowpea are suitable sources of protease inhibitors in breeding exercises for development of transgenic cowpea varieties.
机译：对选定的谷物提取物进行了分析，以分析弯曲变形单胞菌（Anoplocnemiscurvipes），毛白克拉维酵母（Clavigralla tomentosicollis）和小夜蛾（Miperus jaculus）的消化蛋白酶，以鉴定适合the豆害虫的蛋白酶抑制剂的合适来源。实验室生物分析表明，当地大豆品种Kìshí和a豆地方品种NG / SA / 07/0098的谷物均含有有效的抑制剂，尽管前者在三种害虫中均产生较高水平的抑制作用。蛋白酶活性在绒毛棒状杆菌中最高（1.06μmol/ mg），其次是小螺（0.94μmol/ mg）和弯曲曲霉（0.69μmol/ mg），但抑制作用在小ja螺中最高。抑制剂的最佳温度范围是40-90°C，而最佳的抑制活性发生在弱酸性至中性的pH 4-7介质中。当大豆和cow豆提取物与肠道提取物以大豆偏倚的体积比为0.7：0.3和0.8：0.2组合时，害虫中的总蛋白酶被抑制。这种协同功效优于两种商业产品。抑制剂在80°C的温度下可稳定10-50分钟，但在暴露于更高温度的几分钟内会变性。所得结果表明，大豆和are豆是用于转基因development豆品种育种的蛋白酶抑制剂的合适来源。

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