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首页> 外文期刊>African Journal of Biotechnology >Epithelial-mesenchymal transition is associated with increased invasiveness of side population cells from hepatoma SMMC-7721 cells
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Epithelial-mesenchymal transition is associated with increased invasiveness of side population cells from hepatoma SMMC-7721 cells

机译：上皮-间质转化与来自肝癌SMMC-7721细胞的侧群细胞侵袭性增加有关

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To study the?in vitro?invasiveness of side population (SP),?cells purified from hepatoma SMMC-7721 cells and the expressions of epithelial-mesenchymal transition (EMT) markers of SP cells and non-SP (NSP) cells,?fluorescence-activated cell sorting (FACS) was used to sort SP cells and NSP cells from SMMC-7721 cells. The?in vitro?invasiveness of both subpopulations was tested by Transwell chamber assay. Quantitative real-time?reverse transcriptase-polymerase chain reaction (RT-PCR)?and western blot were applied to evaluate the expressions of Vimentin, E-cadherin, N-cadherin and Snail in SP cells and NSP cells, respectively. In Transwell invasion assay, the number of SP cells (66.3 ± 7.4) penetrating the basilar membrane was significantly higher?than?that of NSP cells (27.5 ± 6.5) (P?< 0.01). The mRNA expressions of Vimentin, N-cadherin and Snail was significantly increased in SP cells (9.527-fold, 6.834-fold and 8.173-fold, respectively) when compared with that in NSP cells (P?< 0.01). In contrast, the decrease of E-cadherin mRNA expression in SP cells (5.353-fold) was markedly higher than that in NSP cells (P<0.01). The protein expressions of E-cadherin, Vimentin, N-cadherin and Snail in SP cells were markedly different from those in NSP cells (0.174 ± 0.014 and 0.935 ± 0.012; 1.117 ± 0.012 and 0.314±0.011; 0.975 ± 0.017 and 0.179 ± 0.013; 0.917 ± 0.014 and 0.202 ± 0.013;?P?< 0.01). The results suggest that SP cells from hepatoma SMMC-7721 cells are highly invasive?in vitro?which may be related to the changes in expressions of EMT markers.
机译：为了研究侧群（SP）的体外侵袭性，从肝癌SMMC-7721细胞纯化的细胞以及SP细胞和非SP（NSP）细胞的上皮-间质转化（EMT）标记的表达，荧光活化细胞分选（FACS）用于从SMMC-7721细胞分选SP细胞和NSP细胞。这两个亚群的体外侵袭性均通过Transwell室测定法进行了测试。定量实时逆转录聚合酶链反应（RT-PCR）和western blot方法分别检测Vimentin，E-cadherin，N-cadherin和Snail在SP细胞和NSP细胞中的表达。在Transwell侵袭试验中，穿透基底膜的SP细胞数量（66.3±7.4）明显高于NSP细胞（27.5±6.5）（P <0.01）。与NSP细胞相比，SP细胞中波形蛋白，N-钙黏着蛋白和Snail的mRNA表达显着增加（分别为9.527倍，6.834倍和8.173倍）（P <0.01）。相反，SP细胞中E-钙黏着蛋白mRNA表达的下降（5.353倍）明显高于NSP细胞（P <0.01）。 SP细胞中E-钙粘蛋白，波形蛋白，N-钙粘蛋白和Snail的蛋白质表达与NSP细胞有明显差异（0.174±0.014和0.935±0.012; 1.117±0.012和0.314±0.011; 0.975±0.017和0.179±0.013 ; 0.917±0.014和0.202±0.013;ΔP≤＜0.01）。结果提示肝癌SMMC-7721细胞中的SP细胞具有较高的体外侵袭性，可能与EMT标志物的表达变化有关。

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