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首页> 外文期刊>African Journal of Biotechnology >Molecular characterization of Yucatan tomato phytoplasma (Group 16Sr III)
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Molecular characterization of Yucatan tomato phytoplasma (Group 16Sr III)

机译：犹加敦州番茄植原体（16Sr III组）的分子表征

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摘要

Tomato (Lycopersicon esculentum) is an important vegetable crop in Mexico. Recently, a phytoplasma associated with leaf yellowing and curling, severe stunting and little leaf in tomato plant was identified as Yucatan tomato phytoplasma (16SrIII group). DNAs extracted from tomato leaves with symptoms were examined for the presence of this phytoplasma by nested?polymerase chain reaction (PCR). Positive results were obtained in 44% of samples, yielding an rDNA product of 1.25 kb.?In vitroand?in silico?restriction fragment length polymorphism (RFLP)?patterns obtained with endonucleases?HpaII,?MseI,?RsaI and?TaqI were characteristics of group 16SrIII, according to the classification scheme of phytoplasmas. The pattern with?AluI andHaeIII discriminated between these phytoplasmas and the members of 16SrIII group. Molecular characterization of the causal agent of Yucatan tomato phytoplasma will facilitate the study of this disease’s epidemic aspects and its phytosanitary management. In addition, it will contribute to a greater knowledge of the genetic diversity of phytoplasmas present in Mexico.
机译：番茄（Lycopersicon esculentum）是墨西哥重要的蔬菜作物。最近，番茄叶片中与叶黄化和卷曲，严重发育不良和小叶相关的植物质被鉴定为尤卡坦州的番茄植物质（16SrIII组）。通过巢式聚合酶链反应（PCR）检查从番茄叶片中提取的有症状的DNA，以检查是否存在该植物质体。在44％的样品中获得了阳性结果，产生了1.25 kb的rDNA产物。特征在于，使用核酸内切酶HpaII，MseI，RsaI和TaqI获得的体外和硅限制性片段长度多态性（RFLP）模式是特征。根据植物质原体的分类方案，确定16SrIII组。这些植物质浆体与16SrIII组成员之间具有？AluI和HaeIII的模式。尤卡坦州番茄植原体病原体的分子特征将有助于对该病的流行方面及其植物检疫管理的研究。此外，它将有助于对墨西哥目前存在的植物原质的遗传多样性有更多的了解。

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