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首页> 外文期刊>African Journal of Biotechnology >Bacterial community patterns of municipal water of Sukkur city in different seasons
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Bacterial community patterns of municipal water of Sukkur city in different seasons

机译：不同季节Sukkur市城市用水的细菌群落格局

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The objective of this study was to determine whether there was an association of seasonal variation and bacterial communities of municipal water. The sampling was carried out fortnightly after a flow time of 5 min to eliminate any contaminant present in the mouth of tap in sterilized screw caped 500 ml white glass flasks (Pyrex), containing 0.1 ml of a 1.8% solution of sodium thiosulphate pentahydrate (Na2S2O3.5H2O) per 100 ml of sample. Samples were placed in ice boxes and brought to laboratory within 1 h of collection. Samples were analyzed for bacterial communities using standard microbiological method (membrane filtration technique). The suspected colonies were then further purified and identified using API 20E (BioMerieux) commercial identification kit. Twelve pathogenic bacterial species were isolated and identified from municipal water on conventional and selective media.Their prevalence was higher in?summer season. The average isolation rate was as follows:?Escherichia?coli?69.4%,?Proteus?mirabilis?65.2%,?Providencia?rettgeri?65.2%,Providencia?stuarti?61%,?Klebsiella?oxytoca?54.1%,?Citrobacter?youngae?60%,?Non fermenter species 57%,?Chryseobacterium?meningosepticum?51.3%,?Vibro mimicus39%,?V. cholerae?38%,?Aeromons?hydrphilia?65.2% and?Pseudomonas aeruginosa78%. It is important to mention that water samples were positive for?the?above pathogens throughout the study period (2005 to 2007).?The temperature of water samples was reported highest in July to September and the pH of water samples ranged 7 and 7.8. The bacteriological quality of drinking water under study was very poor. In summer, the isolation rate of bacterial communities was higher than in winter.
机译：这项研究的目的是确定市政水的季节性变化和细菌群落之间是否存在关联。在经过5分钟的流动时间后，每两周进行一次采样，以消除在装有0.1 ml 1.8％硫代硫酸钠五水合物（Na2S2O3）的500毫升带帽旋盖灭菌的白色玻璃烧瓶（Pyrex）中水龙头口中存在的任何污染物。每100毫升样品中加入0.5H2O。将样品放在冰箱中，并在收集后1小时内送至实验室。使用标准微生物学方法（膜过滤技术）分析样品的细菌群落。然后使用API​​ 20E（BioMerieux）商业鉴定试剂盒进一步纯化和鉴定可疑菌落。在常规和选择性介质中，从市政用水中分离并鉴定出12种致病细菌种类。在夏季，它们的流行率较高。平均分离率如下：大肠埃希氏菌为69.4％，奇异变形杆菌为65.2％，Providencia？rettgeri为65.2％，Providencia？stuarti？为61％，克雷伯菌？产妇为54.1％，柠檬酸杆菌为？。幼龄菌占60％，非发酵菌占57％，肠杆菌属细菌占51.3％，拟态弧菌占39％，V。霍乱占38％，嗜气单胞菌占65.2％，铜绿假单胞菌占78％。值得一提的是，在整个研究期间（2005年至2007年），水样均对上述病原体呈阳性。据报道，水样温度在7月至9月最高，pH值在7和7.8之间。研究中的饮用水的细菌学质量非常差。夏季，细菌群落的隔离率高于冬季。

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