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首页> 外文期刊>African Journal of Biotechnology >Effect of mating types on amorpha-4,11-diene production in engineered Saccharomyces cerevisiae
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Effect of mating types on amorpha-4,11-diene production in engineered Saccharomyces cerevisiae

机译：交配类型对酿酒酵母中Amorpha-4,11-二烯产量的影响

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摘要

Amorpha-4,11-diene is the precursor of artemisinin, an antimalarial drug. The effect of yeast mating types on the production of amorpha-4,11-diene was investigated with the aim of improving the yield of the metabolite in engineered?Saccharomyces cerevisiae. A yeast expression vector pYeDP60/GAPDH/ADS harbouring the amorpha-4,11-diene synthase (ADS) gene was transformed into?S. cerevisiae?W303-1A and W303-1B, which showed a and α (alpha) mating types, respectively. We also investigated the effects of four variables (carbon source, glucose concentration, nitrogen source and pH) on the fermentative production of amorpha-4,11-diene by the different mating types. Only slight differences were observed in the yields of amorpha-4,11-diene produced by the engineered yeasts, W303A[ADS] and W303B[ADS]. There were also no significant differences in the amounts of amorpha-4,11-diene produced under various growth conditions. The data generated in this study suggest that mating types of?S. cerevisiae?had no influence on amorpha-4,11-diene production levels and, therefore, either of the two mating types could be used as the parent strain of engineered yeasts.
机译：Amorpha-4,11-diene是抗疟药青蒿素的前体。为了提高工程酿酒酵母中代谢产物的收率，研究了酵母交配类型对4,11-amorpha非晶态产生的影响。将具有amorpha-4，11-二烯合酶（ADS）基因的酵母表达载体pYeDP60 / GAPDH / ADS转化为ΔS。啤酒酵母W303-1A和W303-1B，分别显示出α和α（α）交配类型。我们还研究了四个变量（碳源，葡萄糖浓度，氮源和pH）对不同交配类型发酵生产amorpha-4,11-diene的影响。工程酵母W303A [ADS]和W303B [ADS]产生的amorpha-4,11-二烯的收率仅观察到微小差异。在各种生长条件下，生产的4,11-二烯非晶质的量也没有显着差异。在这项研究中产生的数据表明，交配类型为？S。酿酒酵母对魔芋4,11-二烯的生产水平没有影响，因此，两种交配类型中的任何一种都可以用作工程酵母的亲本菌株。

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