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A more improved protocol for in vitro shoot organogenesis in daylily (Hemerocallis sp.)

机译:黄花菜(Hemerocallis sp。)体外芽器官发生的一种改进方法

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Daylily is a crop of landscape, nutritional, and medicinal importance. Despite relative progress, daylily is one of the least explored crops in tissue culture, partly, because of its low responsiveness for?in vitro?organogenesis. This study reports a more efficient one-step protocol for shoot organogenesis. Leaf, flower, flower bud, stem, and root tissues were investigated for shoot organogenesis after different growth regulator treatments on Murashige and Skoog medium. Three out of four of the daylily formed shoots. The variety 'Summer Echoes' was the best of all the other varieties with the greatest average shoots per explant (118) and shooting explant rate 87% when 6-benzyl-aminopurine (BA) and kinetin were used singly. This protocol is the most improved, over the current ones, for shoot organogenesis in daylily. The plant grew normally in the greenhouse.
机译:黄花菜是一种具有景观,营养和药用价值的作物。尽管取得了相对的进步,但黄花菜还是组织培养中探索最少的农作物之一,部分原因是其对体外器官发生的反应性低。这项研究报告了芽器官发生的一种更有效的一步协议。在Murashige和Skoog培养基上不同生长调节剂处理后,研究了叶,花,花蕾,茎和根组织的芽器官发生。黄花菜中有四分之三的芽。 “夏季回声”品种是所有其他品种中最好的品种,每株外植体的平均芽数最高(118),当单独使用6-苄基氨基嘌呤(BA)和激动素时,出芽率高达87%。与目前相比,该协议是针对黄花菜器官发生的最完善的协议。该植物通常在温室中生长。

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