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首页> 外文期刊>African Journal of Biotechnology >Genetic analysis of japonica x indica recombinant inbred lines and characterization of major fragrance gene by microsatellite markers
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Genetic analysis of japonica x indica recombinant inbred lines and characterization of major fragrance gene by microsatellite markers

机译：粳稻重组自交系的遗传分析及主要香味基因的微卫星标记鉴定

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Traditional basmati rice varieties are very low yielding due to their tendency to lodging and increasing susceptibility to diseases. To improve the characters of basmati rice variety and study the inheritance of various physio-morphological and quality characters,?F5?population comprising of 204 lines from the cross between NPT II (non-aromatic,?japonica) and Taraori Basmati or HBC19 (aromatic,?indica), were evaluated. Ample amount of genetic variability was observed for the characters plant height, tillers per plant, kernel length, kernel breath and L/B ratio. The grain yield/plant showed positive correlation with productive tiller/plant and test weight. Path coefficient analysis showed that the productive tiller/plant and test weight contribute to grain yield/ plant through direct effect. The parent off-spring regression was high for all the characters under study suggesting improvement of these characters by mere selection. Based on divergence study, 204 lines were categorized in seven clusters whereas parents were grouped in different clusters. Molecular restricted selection using specific SSR markers with depicting high correlation with aroma could offer great promise to select high yielding rice among high aroma lines. A total of 54 randomly selected F5?plants were subjected to SSR marker analysis using SSR markers. The F5?plants had an allele from either of the two parental lines (homozygous condition) or alleles from both the parental rice varieties (heterozygous condition). At some SSR loci, new/recombinant alleles were observed, which indicate the active recombination between genomes of two rice varieties and can be used for linkage mapping once complete homozygosity is achieved. SSR allelic profile based on two dimensional principal component analysis demonstrated high level of diversity among parents and F5?plants spread between them.
机译：传统的印度香米品种由于易于倒伏和对疾病的易感性高而单产很低。为了改善印度香米的性状并研究各种生理形态和品质性状的遗传，从NPT II（非芳香族，日本粳稻）和Taraori Basmati或HBC19（芳香族）之间杂交的204系组成的“ F5”群体，？indica），进行了评估。对于植物高度，单株分till，籽粒长度，籽粒呼吸和L / B比，观察到大量的遗传变异。谷物产量/植物与生产性分//植物和试验重量呈正相关。路径系数分析表明，生产性分plant /植株和表观重量通过直接作用对谷物产量/植株有贡献。对于所有正在研究的字符，其父代后代回归均很高，表明仅通过选择即可改善这些字符。根据差异性研究，将204条品系分为7个类群，而将亲本分为不同的类群。使用具有与香气高度相关性的特定SSR标记进行分子限制性选择，可以为在高香气系中选择高产水稻提供很大的希望。使用SSR标记对总共54个随机选择的F5？植物进行SSR标记分析。 F5植物有两个亲本系中的一个等位基因（纯合条件）或两个亲本水稻品种的等位基因（杂合条件）。在一些SSR基因座上，观察到了新的/重组等位基因，这表明两个水稻品种的基因组之间发生了有效的重组，一旦实现了完全纯合，就可以用于连锁作图。基于二维主成分分析的SSR等位基因谱显示出亲本之间的高度多样性，F5植物在它们之间传播。

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