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首页> 外文期刊>African Journal of Biotechnology >Gene expression associated with the biosynthetic pathway of abscisic acid, gibberellin and ethylene during lettuce seed development
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Gene expression associated with the biosynthetic pathway of abscisic acid, gibberellin and ethylene during lettuce seed development

机译：生菜种子发育过程中脱落酸，赤霉素和乙烯的生物合成途径相关的基因表达

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摘要

Seed development may be divided up into embryonic morphogenesis and seed maturation, while diverse genes were connected and disconnected in order to bring about such processes.? Features such as desiccation tolerance, dormancy and germination capacity are acquired throughout development and regulated by the presence and interaction of phytohormones such as abscisic acid (ABA), gibberellin (GA) and ethylene. The purpose of this study was to analyze the expression of genes connected with ABA, GA and ethylene biosynthesis during the development of lettuce seeds. For that reason, expression of the?LsNCED, LsGAox?and?ACO-B?genes was evaluated by means of the real time polymerase chain reaction (PCR) technique in seeds from seven lettuce cultivars harvested at the maturation stages of 5, 10, 15 and 20 days after anthesis. It may be concluded that expression of the?LsNCED?gene was related to the imposition of dormancy at the final stages of development; expression of the?LsGA3ox?gene occurred at the initial stages of development; the expression of theACO-B?was greater in the initial (5 DAA) and late (20 DAA) stages during development, inducing germination. The expression profile of the?LsNCED, LsGA3oxand?ACO-B?genes was dependent on the cultivar.
机译：种子的发育可以分为胚胎形态发生和种子成熟，而各种基因则通过连接和断开来实现这种过程。在整个发育过程中都获得了诸如耐干燥性，休眠和发芽能力等特性，并受脱落酸（ABA），赤霉素（GA）和乙烯等植物激素的存在和相互作用的调节。本研究的目的是分析莴苣种子发育过程中与ABA，GA和乙烯生物合成相关的基因的表达。因此，通过实时聚合酶链反应（PCR）技术，评估了在5、10、10，花后15和20天。可以得出结论，“ LsNCED”基因的表达与在发育的最后阶段施加休眠有关。 LsGA3ox基因的表达发生在发育的初始阶段。在发育的最初阶段（5 DAA）和后期阶段（20 DAA），ACO-Bα的表达较高，从而诱导发芽。 LsNCED，LsGA3oxandACO-Bβ基因的表达谱取决于品种。

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