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Micropropagation of Plectranthus edulis (Vatke) Agnew from meristem cultur

机译:从分生组织培养微生食香(Vatke)Agnew

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Plectranthus edulis (Vatke) Agnew is a tuber-bearing food crop in Ethiopia. However, its productivity is hampered by shortage of pathogen-free planting materials. Therefore, the objective of this study was to develop micropropagation protocol for this plant using meristem to produce clean planting materials. Mersitems were collected from apical and axillary shoots from Holeta and Welayta areas and cultured on MS medium containing gibberellic acid (GA3) (1.0 mg l-1), α-naphthalene acetic acid (NAA) (0.1 mg l-1) in combination with 6-benzylaminopurine (BAP) (0.1, 0.5, 1.0, 2.0 and 5.0 mg l-1). Shoots were multiplied on MS medium containing 0.1, 0.5, or 1.0 mg l-1 of BAP or 0.5, 1.0, 2.0 or 3.0 mg l-1 of Kinetin alone or their combination with 0.05 or 0.1 mg l-1 NAA. In vitro and ex vitro rooting was performed using different types of auxins followed by acclimatization. The highest percentage of shoots initiated from collected mersitem at Holeta (73%) was obtained on MS medium containing 1.0 mg l-1 BAP, 1.0 mg l-1 GA3 and 0.1 mg l-1 NAA. The highest shoot number explants-1 (7.2) was obtained on MS medium containing 1.0 mg l-1 Kinetin and 0.1 mg l-1 NAA, whereas the highest root number shoot-1 (6.2) was obtained from ex vitro. All plants derived from Holeta and 96.7% of those from the Welayta survived after acclimatization. These results provided rapid and reproducible conditions for propagation of relatively pathogen-free planting material of this plant.
机译:Plectranthus edulis(Vatke)Agnew是埃塞俄比亚的一种块茎类粮食作物。但是,由于缺乏无病原体的种植材料,其生产率受到了阻碍。因此,本研究的目的是开发使用分生组织生产清洁种植材料的该植物的微繁殖方案。从Holeta和Welayta地区的根尖和腋生芽中收集到梅虫素,并在含有赤霉素(GA3)(1.0 mg l-1),α-萘乙酸(NAA)(0.1 mg l-1)和6-苄氨基嘌呤(BAP)(0.1、0.5、1.0、2.0和5.0 mg 1-1)。在含有0.1、0.5或1.0 mg l-1的BAP或0.5、1.0、2.0或3.0 mg l-1的Kinetin或其与0.05或0.1 mg 1-1 NAA的组合的MS培养基上繁殖芽。使用不同类型的生长素进行体外和离体生根,然后进行驯化。在含有1.0 mg l-1 BAP,1.0 mg l-1 GA3和0.1 mg l-1 NAA的MS培养基上,在Holeta收集到的最多的美生菌所产生的芽的百分率最高(73%)。在含有1.0 mg -1 Kinetin和0.1 mg 1-1 NAA的MS培养基上获得最高的芽数外植体-1(7.2),而从离体获得最高的根数shoot-1(6.2)。适应后,所有来自霍乐塔的植物和来自Welayta的植物的96.7%都可以存活。这些结果为该植物的相对无病原体的种植材料的繁殖提供了快速和可重复的条件。

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