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首页> 外文期刊>African Journal of Biotechnology >Addressing the issue of horizontal gene transfer from a diet containing genetically modified components into rat tissues
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Addressing the issue of horizontal gene transfer from a diet containing genetically modified components into rat tissues

机译：解决从含有转基因成分的饮食向大鼠组织中水平基因转移的问题

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Genetically modified (GM) food crops are considered to have the potential of providing food security especially in developing countries. Scientists have raised concern over the hazards associated with the consumption of genetically modified organisms (GMOs). One of these hazards, which have great controversy reports, is the possible horizontal gene transfer from GM-food or feed to human or animal tissues. Many researches were conducted to investigate the presence of some transgenic sequences in animal tissues fed on GM- crops. Many of the inserted genes in the GM-crops are under the control of the promoter of the Cauliflower mosaic virus (CaMVP35S) and produce insecticidal proteins. Health hazards are suggested to accompany the ingestion of this promoter. CaMVP35S can function in a wide range of organisms (plants and animals). It has also been demonstrated that the CaMV-P35S promoter sequence can convert an adjacent tissue- and organ-specific gene promoter into a globally active promoter. The present work was conducted to evaluate the possibility of horizontal gene transfer from a diet containing DNA segments from Cauliflower mosaic virus -35S promoter (CaMVP-35S) to the cells of different organs of rats fed for three months on diets containing genetically modified components. Analysis of the results revealed that: 1) ingested fragments from the CaMV-35S promoter incorporated into blood, liver, and brain tissues of experimental rats, 2) The total mean of transfer of GM target sequences increased significantly by increasing the feeding durations, and 3) The affinity of different transgenic fragments from the ingested GM-diet, to be incorporated into the different tissues of rats varied from one target sequence to the other.
机译：转基因（GM）粮食作物被认为具有提供粮食安全的潜力，尤其是在发展中国家。科学家对与食用转基因生物（GMO）相关的危害提出了关注。有大量争议性报道的这些危害之一是可能从转基因食品或饲料向人类或动物组织的水平基因转移。进行了许多研究以调查以转基因作物为食的动物组织中某些转基因序列的存在。 GM农作物中许多插入的基因在花椰菜花叶病毒（CaMVP35S）的启动子的控制下，并产生杀虫蛋白。建议摄入健康危害健康。 CaMVP35S可以在多种生物（植物和动物）中起作用。还已经证明，CaMV-P35S启动子序列可以将相邻的组织和器官特异性基因启动子转化为全局活性启动子。进行本工作以评估水平基因从含有花椰菜花叶病毒-35S启动子（CaMVP-35S）的DNA片段的饮食向含有转基因成分的饮食喂养三个月的大鼠不同器官细胞水平转移的可能性。结果分析表明：1）从CaMV-35S启动子摄取的片段掺入了实验大鼠的血液，肝脏和脑组织中; 2）GM靶序列转移的总平均值随着进食时间的延长而显着增加，并且3）从摄入的GM饮食中摄取的不同转基因片段的亲和力，将其掺入大鼠的不同组织中，从一个靶序列到另一个靶序列都不同。

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