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首页> 外文期刊>African Journal of Biotechnology >Simulated inhibitory effects of typical byproducts of biomass pretreatment process on the viability of Saccharomyces cerevisiae and bioethanol production yield
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Simulated inhibitory effects of typical byproducts of biomass pretreatment process on the viability of Saccharomyces cerevisiae and bioethanol production yield

机译：模拟生物质预处理过程中典型副产物对酿酒酵母活力和生物乙醇产量的抑制作用

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The abundance of second generation feedstock reinforces the consideration of biofuel over fossil fuel, as bioethanol can be produced from lignocellulosic materials. However, the pretreatment required for oxidation of lignocellulose into hexose often results in the production of inhibitors likely to impede the activity of Saccharomyces cerevisiae during bioethanol production. This study aimed to investigate the comparative inhibitory effects of acetic acid and vanillin on the viability of S. cerevisiae and the production yield of bioethanol. Different concentrations of inhibitors were spiked in the fermentation broth then the production of bioethanol monitored overtime and correlated with cell viability. The results showed that the inhibition of S. cerevisiae by vanillin is more potent compared to acetic acid; however the reduction of bioethanol yield after 12 h was more pronounced with acetic acid (42.8% reduction) than with vanillin (33.3% reduction) which was ascribed to the simultaneous production of weak acids during the fermentation process. The viability test has shown that in the presence of lower concentrations of inhibitors, S. cerevisiae can adapt for the first 12 h of fermentation and then may improve ethanol production yield overtime. At lower concentrations (2 g/l vanillin and 4 g/l acetic acid) the effect of inhibitors on the viability of S. cerevisiae and ethanol productivity does not last and can be overcome by the adaptation of the yeast. However, the presence of higher concentrations (4 g/l vanillin and 6 g/l acetic acid) results to nearly total inhibition of bioethanol production and the remediation of such effect may therefore require a detoxification process.
机译：第二代原料的丰富性增强了对生物燃料而不是化石燃料的考虑，因为生物乙醇可以由木质纤维素材料生产。然而，将木质纤维素氧化为己糖所需的预处理通常导致抑制剂的产生，该抑制剂可能会在生物乙醇生产过程中阻碍啤酒酵母的活性。本研究旨在研究乙酸和香兰素对啤酒酵母活力和生物乙醇产量的比较抑制作用。将不同浓度的抑制剂掺入发酵液中，然后随时间监测生物乙醇的产生，并与细胞活力相关。结果表明，与乙酸相比，香兰素对啤酒酵母的抑制作用更强。然而，乙酸（减少42.8％）比香草醛（减少33.3％）在12小时后的生物乙醇产量降低更为明显，这归因于发酵过程中同时产生弱酸。生存力测试表明，在抑制剂浓度较低的情况下，酿酒酵母可以适应发酵的前12小时，然后可以随着时间的推移提高乙醇的产量。在较低浓度（2 g / l香兰素和4 g / l乙酸）下，抑制剂对酿酒酵母活力和乙醇生产率的影响不会持续，并且可以通过酵母的适应来克服。但是，较高浓度（4 g / l香兰素和6 g / l乙酸）的存在导致生物乙醇生产的几乎全部抑制，因此，要补救这种作用可能需要进行解毒过程。

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