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首页> 外文期刊>African Journal of Biotechnology >A commercial micropropagation protocol for virupakshi (AAB) banana via apical meristem
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A commercial micropropagation protocol for virupakshi (AAB) banana via apical meristem

机译：通过顶端分生组织对virupakshi（AAB）香蕉进行商业化微繁殖的方案

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摘要

In vitro micropropagation of banana (Musa spp.) cv.virupakshi (Hillbanana) was studied. Suckers were collected from the germ plasm block of Jain R&D (originally established from the suckers from Palani Hills, Tamil Nadu) during summer. The sucker surface sterilized with 1% NaOCl for 30 min gave 100% survival without any contamination. Apical meristems that were isolated and cultured on MS based media supplemented with benzylaminopurine (BAP) 10.0 mg/l and IAA1.0 mg/l gave higher number of shoots (134.3 shoots/explant) within168 days (24 weeks). Kinetin 2.0 mg/l and NAA0.5 mg/l gave early rooting in just five days with 6.6 roots per plant. Observations were recorded after every four weeks up to six sub-culturing. Acclimatization was done in poly house, followed by shade house under 50% light conditions. The hardened plants when shifted to the field showed luxurious growth. The regenerated micro propagated banana plants were tested for genetic uniformity through 13 inter simple sequence repeat (ISSR) markers recommended by NCS-TCP, DBT. Profiles obtained by all the three ISSR primers namely, 834, 840 and 850, respectively exhibited similar banding patterns, which revealed the existence of genetic uniformity in micro- propagated plants.
机译：研究了香蕉（Musa spp。）cv.virupakshi（Hillbanana）的体外微繁。在夏季，从Jain R＆D的种质模块（最初从泰米尔纳德邦Palani Hills的吸盘建立）中收集吸盘。用1％NaOCl消毒的抽油杆表面经过30分钟消毒后，存活率达到100％，没有任何污染。在补充有10.0 mg / l和IAA1.0 mg / l的苄基氨基嘌呤（BAP）的MS基培养基上分离并培养的根尖分生组织在168天（24周）内有较高数量的芽（134.3芽/植株）。 Kinetin 2.0 mg / l和NAA0.5 mg / l在短短五天内就可以早生，每株植株有6.6个根。每四周记录一次观察结果，最多进行六次亚培养。在棚屋中进行驯化，然后在50％的光照条件下遮阴。转移到田间的硬化植物生长迅速。通过NCS-TCP，DBT推荐的13个简单序列重复（ISSR）标记，测试了再生的微繁殖香蕉植株的遗传一致性。所有三种ISSR引物，即834、840和850所获得的图谱分别显示出相似的条带模式，这表明在微繁殖植物中存在遗传均匀性。

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