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首页> 外文期刊>African Journal of Biotechnology >In vitro culture and plant regeneration derived from ray florets of Chrysanthemum morifolium
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In vitro culture and plant regeneration derived from ray florets of Chrysanthemum morifolium

机译：菊花射线小花的离体培养和植株再生

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摘要

Nine cultivars of?Chrysanthemum?morifolium?were screened using the ray floret explants to determine the capability for plant regeneration on four media protocols and subsequently to find out the best genotype source linked with the optimum medium conditions for the high potentiality of shoot formation. The results indicated that all?in vitro?culture traits were highly significantly influenced by the differences in genotypes, medium protocols and their interaction. The percentage of explants which developed calli ranged from 73.83% “Ping Pong” to 25.67% “Palisade White” among the cultivars across the four medium protocols with an average of 48.28%. The highest percentage of embryogenic callus, shoot formation and mean value of shoot length was produced by cultivar "Delistar White" when calli were differentiated on medium protocol B. The medium protocol B showed the greatest potential for shoot length across the cultivars and it was significantly superior to all other medium protocols except the medium protocol A. The present study indicated that the medium protocol “B” and then "A" appear to be the best protocols for plant regeneration. The cultivar "Delistar White" with the medium protocols B and then A, could be successfully utilized for further?in vitro?mutagenesis investigations.
机译：使用射线小花外植体筛选了9个菊花形变种，以确定四种培养基方案下植物的再生能力，随后找出与最佳培养基条件相关的最佳基因型来源，从而具有较高的芽形成潜力。结果表明，基因型，培养基规程及其相互作用的差异对所有体外培养性状均具有显着影响。在四个培养基方案中，形成愈伤组织的外植体百分比在“乒乓”中占73.83％，在“栅栏白”中占25.67％。当在培养基方案B上对愈伤组织进行分化时，品种“ Delistar White”产生的胚性愈伤组织，芽形成和芽长度的平均值最高。培养基方案B在整个品种上显示出最大的芽长潜力，并且显着优于除培养基协议A以外的所有其他培养基协议。本研究表明，培养基协议“ B”然后是“ A”似乎是植物再生的最佳协议。带有B和A培养基的“ Delistar White”品种可以成功地用于进一步的体外诱变研究。

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