首页> 外文期刊>African Journal of Biotechnology >Complete nucleotide sequence and organization of the mitogenome of endangered Eumenis autonoe (Lepidoptera: Nymphalidae)
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Complete nucleotide sequence and organization of the mitogenome of endangered Eumenis autonoe (Lepidoptera: Nymphalidae)

机译:濒危Eumenis autonoe(鳞翅目:Nymphalidae)有丝分裂基因组的完整核苷酸序列和组织

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Eumenis autonoe,?a member of the?lepidopteran family, Nymphalidae (superfamily Papilionoidea) is an endangered species and is found only on one isolated remote island Jeju in South Korea, on Halla Mt, at altitudes higher than 1,400 m. In this study, the complete mitochondrial genome (mitogenome) of?E. autonoe?was reported.?The?15,489-bp long?E. autonoe?genome evidenced the typical gene content found in animal mitogenomes, and harbors?the?gene arrangement identical to all other sequenced lepidopteran insects, which differs from the most common type found in insects, due to the movement of tRNAMet?to a position 5’-upstream of tRNAIle. As has been observed in many other lepidopteran insects, no typical ATN codon for the COI gene is available. Thus, we also designated the CGA (arginine) found at the beginning of the COI gene as a lepidopteran COI starter, in accordance with previous suggestions. The 678 bp long A + T-rich region, which is second longest in sequenced lepidopteran insects, harbored 10 identical 27 bp long tandem repeats plus one 13 - bp long incomplete final repeat. Such a repeat sequence has been, thus far, only rarely detected in lepidopteran mitogenomes. The?E. autonoe?A + T-rich region harbored a poly-T stretch of 19 bp and a conserved ATAGA motif located at the end of the region, which have been suggested to function as structural signals for minor-strand mtDNA replication.?Phylogenetic reconstructionusing the concatenated 13 amino acid and nucleotide sequences of the protein-coding genes (PCGs) consistently supported a close relationship betweenBombycoidea and Geometroidea?among six available lepidopteran superfamilies (Tortricoidea,?Pyraloidea, Papilionoidea, Bombycoidea, Geometroidea and Noctuoidea). Among the true butterflies (Pieridae, Nymphalidae, Lycaenidae andPapilionidae), a closer relationship between Lycaenidae and Pieridae, excluding Nymphalidae was consistently concluded to exist, although this result deviated from the traditional view.
机译:Eumenis autonoe是鳞翅目家族的一员,Nymphalidae(超家族Papilionoidea)是一种濒临灭绝的物种,仅在韩国海拔1400 m以上的汉拿山一个偏僻的济州岛上发现。在这项研究中,?E的完整线粒体基因组(有丝分裂基因组)。报告了autonoe。长15,489-bp。自主基因组证明了在动物有丝分裂基因组中发现的典型基因含量,并且其基因排列与所有其他测序的鳞翅目昆虫相同,这与昆虫中最常见的类型不同,这是由于tRNAMet移至第5位引起的。在tRNAIle上游。正如在许多其他鳞翅目昆虫中所观察到的那样,没有用于COI基因的典型ATN密码子。因此,根据先前的建议,我们还将在COI基因开头发现的CGA(精氨酸)指定为鳞翅目COI起始物。 678 bp长的富含A + T的区域,是测序的鳞翅目昆虫中第二长的区域,具有10个相同的27 bp长的串联重复序列,以及一个13 bp的不完整最终重复序列。迄今为止,这种重复序列在鳞翅目有丝分裂基因组中很少被发现。 ?E。 Autonoe?A + T丰富的区域在该区域的末端具有一个19 bp的poly-T片段和一个保守的ATAGA基序,这被认为可以作为短链mtDNA复制的结构信号。蛋白质编码基因(PCG)的13个氨基酸和核苷酸序列连接在一起,始终支持在6个可用鳞翅目超家族(Tortricoidea,?Pyraloidea,Papilionoidea,Bombycoidea,Geometroidea和Noctuoidea)中,Bombycoidea和Geometroidea?之间存在密切的关系。在真正的蝴蝶(Pieridae,Nymphalidae,Lycaenidae和Papilionidae)中,Lycaenidae和Pieridae之间(除Nymphalidae以外)之间一直存在着更紧密的联系,尽管这一结果偏离了传统观点。

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