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首页> 外文期刊>African Journal of Biotechnology >Morphological and molecular screening of rice accessions for salt tolerance
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Morphological and molecular screening of rice accessions for salt tolerance

机译：水稻耐盐性的形态和分子筛选

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The ever-increasing demand for rice raises the need to increase productivity by using salt tolerant varieties on saline soils. In this study, 12 rice accessions were screened for tolerance to salt at seedling stage using morphological and molecular methods. The study was carried out in a hydroponic system using Hoagland solution. Scoring was done using the modified standard evaluation score (SES) system after 14 days of treatment. Salt tolerance indices were estimated from shoot length, root length and total biomass. For molecular studies, ten Simple Sequence Repeats (SSR) primers linked with salt tolerance Quantitative Trait Loci (QTL) were used. Results showed greater reductions in biomass and shoot growth of susceptible accessions compared to the tolerant ones. The effect of salt stress on root length showed variability among accessions as well as concentrations. UPIA 1, UPIA 2, FARO 52, FARO 61, TOG 5681 and FARO 44 had similar banding patterns with POKALLI (check variety) suggesting that they may contain salt tolerance genes. Of these accessions, only POKALLI and UPIA 2 survived all levels of salt concentration tested and thus got the highest SES score of one. Both accessions also had the highest overall mean salt tolerance indices. In conclusion, based on SES scores, salt tolerance indices and SSR data, POKALLI and UPIA 2 were identified as highly tolerant, FARO61, FARO 52, UPIA 1 and TOG 5980A as tolerant while WITA 12, CG12, TOG1670, TOG 5681 and TOG 5485 were highly susceptible. Such information will be useful in the selection of parents as breeding lines for salt tolerance.
机译：对稻米的需求不断增长，因此需要在盐渍土上使用耐盐品种来提高生产力。在这项研究中，使用形态学和分子学方法筛选了12个水稻种苗在苗期对盐的耐受性。该研究是在采用Hoagland溶液的水培系统中进行的。治疗14天后，使用改良的标准评估评分（SES）系统进行评分。根据枝条长度，根长和总生物量估算耐盐指数。为了进行分子研究，使用了十个与耐盐定量性状位点（QTL）连接的简单重复序列（SSR）引物。结果表明，与耐性种质相比，敏感种质的生物量减少和枝条生长更大。盐胁迫对根长的影响显示出种质之间以及浓度上的差异。 UPIA 1，UPIA 2，FARO 52，FARO 61，TOG 5681和FARO 44具有与POKALLI（检查品种）相似的条带模式，表明它们可能包含耐盐基因。在这些种质中，只有POKALLI和UPIA 2在所有盐浓度水平下均能存活，因此SES得分最高，为1。两种登录品的总体平均耐盐性指数也最高。总之，根据SES分数，耐盐性指数和SSR数据，可以确定POKALLI和UPIA 2具有高度耐受性，FARO61，FARO 52，UPIA 1和TOG 5980A具有耐受性，而WITA 12，CG12，TOG1670，TOG 5681和TOG 5485高度易感。这些信息对于选择父母作为耐盐育种系很有用。

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