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首页> 外文期刊>African Journal of Biotechnology >Calcium chloride improve ethanol production in recombinant Zymomonas mobilis
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Calcium chloride improve ethanol production in recombinant Zymomonas mobilis

机译：氯化钙改善运动发酵单胞菌中乙醇的生产

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摘要

The T7-expression system has been very useful for protein expression inEscherichia coli. Here, a T7-expression transposon was constructed, which allowed simple construction of T7-expression?Zymomonas mobilis. This transposon contained the T7 RNA polymerase being driven by the?gap?promoter from?Z. mobilis.?The T7-expression?fadK?genomes were introduced into?Z. mobilisATCC 31821 in order to increase ethanol production. The recombinant bacteria were named as?Z.M.F-1,?Z.M.F-2,?Z.M.F-3,?and Z.M.F-4. However,?Z.M.F-4?had the highest ability of?producing?ethanol?by selection. Compared with?Z. mobilis?ATCC 3182, there was 7% increase in ethanol production for?Z.M.F-4?with corn hydrolyaztes as fermentation medium. The 16 mmolL-1?supplement of calcium chloride could significantly improve the ethanol production. This was also clearly demonstrated by a variety of kinetic parameter values over time in?Z.M.F-4?under high sugar osmotic stress. Calcium chloride not only increased the fermentation ability but also improved the stability of cell membrane.
机译：T7表达系统对于大肠杆菌中的蛋白质表达非常有用。在这里，构建了一个表达T7的转座子，可以简单地构建表达T7的运动发酵单胞菌。该转座子包含T7 RNA聚合酶，该T7 RNA聚合酶由来自？Z的“缺口”启动子驱动。将T7表达的fadK基因组导入Z。 mobilisATCC 31821，以增加乙醇产量。重组细菌分别命名为“ Z.M.F-1”，“ Z.M.F-2”，“ Z.M.F-3”和“ Z.M.F-4”。然而，Z.M.F-4具有通过选择“生产”乙醇的最高能力。与？Z用玉米水解产物作为发酵培养基，使“ Z.M.F-4”的乙醇产量提高了7％。补充16 mmolL-1？的氯化钙可以显着提高乙醇的产量。高糖渗透压下在Z.M.F-4中随时间变化的各种动力学参数值也清楚地证明了这一点。氯化钙不仅提高了发酵能力，而且提高了细胞膜的稳定性。

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