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Study on relationship between microsatellite polymorphism and producing ability on litter size trait of Hu sheep in China

机译:中国Hu绵羊微卫星多态性与产仔性状关系的研究

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Four microsatellite loci (OarAE101, BM1329, BM143 and OarHH55) linked to FecB gene on chromosome 6 and one microsatellite locus (OarHH55) on chromosome 4 were selected to study their correlation with litter size of Hu sheep breed. The results showed that the average polymorphism information content (PIC), heterozygosity (He) and effective allele number (Ne) were 0.7214, 0.7558 and 4.4094, respectively, and all 5 microsatellite loci reached high polymorphism (PIC?>0.5), and the five microsatellite loci can be used for genetic diversity evaluation in Hu sheep breed. The result of variance analysis showed that there were extreme significant differences among the different genotypes for the producing ability of litter size in OarAE101 locus and BM143 locus (P??0.05). Choosing by combining producing ability and special gene (gene type) will play an great role in improving selecting accuracy and breeding progress, and it is a wonderful and new analyzed thought in repeatable characters with low heritability which is affected by measuring frequencies. And this study will have an important role in MAS (marker-assistant selection) and molecular breeding in Hu sheep in future.
机译:选择4个与6号染色体上的FecB基因连锁的微卫星基因座(OarAE101,BM1329,BM143和OarHH55)和4个染色体上的1个微卫星基因座(OarHH55),以研究它们与Hu羊品种凋落物大小的相关性。结果表明,平均多态性信息含量(PIC),杂合度(He)和有效等位基因数(Ne)分别为0.7214、0.7558和4.4094,并且所有5个微卫星基因座均达到高多态性(PIC≥0.5),且5个微卫星基因座可用于胡羊品种的遗传多样性评估。方差分析结果表明,不同基因型在OarAE101基因座和BM143基因座上产仔数的产生能力存在极显着差异(P≥0.05)。结合生产能力和特殊基因(基因类型)的选择将在提高选择准确性和育种进度方面发挥巨大作用,这是一种受频率影响而可遗传性低的可重复性的奇妙而新颖的分析思想。这项研究将在今后的胡羊MAS(标记辅助选择)和分子育种中发挥重要作用。

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