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首页> 外文期刊>African Journal of Biotechnology >Novel single nucleotide polymorphisms (SNPs) of the bovine STAT4 gene and their associations with production traits in Chinese Holstein cattle
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Novel single nucleotide polymorphisms (SNPs) of the bovine STAT4 gene and their associations with production traits in Chinese Holstein cattle

机译：牛STAT4基因的新型单核苷酸多态性（SNPs）及其与中国荷斯坦牛的生产性状的关联

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摘要

Signal transducer and activator of transcription 4 (STAT4) belongs to the STATs family which may play important roles in the activation of milk protein genes and the development of?mammary glands.?Four?novel single nucleotide polymorphisms (SNPs) (g.2624A>C ss175327225, g.60330A>G ss175327226, g.63823G>C ss175327227, g.66912C>T ss175327228) of the?STAT4?gene were investigated in 966 cattle of three breeds (Chinese holstein, Luxi yellow and Bohai black cattle) in China by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP), PCR-restriction fragment length polymorphism (RFLP) and DNA sequencing methods.?The SNP (g.60330A>G ss175327226) could be genotyped into three genotypes (AA, AG and GG) with PCR-RFLP using?Msp?I?and?another SNP (g.66912C>T ss175327228) only into two genotypes (CC and TC) by PCR-SSCP. The allelic frequencies of the SNP (g.60330A>G) were different among the three different cattle breeds (P G) markedly affected the 305 d matured equivalency (P T) had no significant relationship with the milk production traits (P > 0.05). Together, the SNP (g.60330A>G) of the?STAT4?gene may serve as a molecular marker for the differentiation of various cattle populations and selection of the milk yield and fat content in bovine breeding program.
机译：信号转导子和转录激活子4（STAT4）属于STATs家族，在乳蛋白基因的激活和乳腺的发育中可能起重要作用。四个新的单核苷酸多态性（SNP）（g.2624A> STAT4？基因的C ss175327225，g.60330A> G ss175327226，g.63823G> C ss175327227，g.66912C> T ss175327228）在以下三个品种的966头牛（荷斯坦，Lux西黄和渤海黑牛）中进行了调查。中国采用聚合酶链反应-单链构象多态性（PCR-SSCP），PCR-限制性片段长度多态性（RFLP）和DNA测序方法。？SNP（g.60330A> G ss175327226）可分为三种基因型（AA） ，使用Msp？I？和另一个SNP（g.66912C> T ss175327228）通过PCR-RFLP进行PCR-RFLP分析，只能通过PCR-SSCP分为两种基因型（CC和TC）。在三个不同的牛品种（PG）中，SNP的等位基因频率（g.60330A> G）均不同，显着影响305 d成熟当量（PT）与产奶性状无显着关系（P> 0.05）。 STAT4β基因的SNP（g.60330A> G）一起可以作为分子标记，用于在牛育种计划中区分各种牛群以及选择牛奶产量和脂肪含量。

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