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首页> 外文期刊>African Journal of Biotechnology >Isolation of two Kocuria species capable of growing on various polycyclic aromatic hydrocarbons
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Isolation of two Kocuria species capable of growing on various polycyclic aromatic hydrocarbons

机译：能够在各种多环芳烃上生长的两种Kocuria物种的分离

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Different samples collected from crude oil contaminated beach were enriched for isolation of bacterial strains capable of growing on naphthalene, phenanthrene and fluoranthene. Respiratory reduction of?WST-1{4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate} to a colored formazan?showed?that one isolated strain CMG2028, identified as?Kocuria flava?by 16s rRNA,?grew on naphthalene and phenanthrene while CMG2042, identified as?Kocuria rosea?grew on all three?polycyclic aromatic hydrocarbons?(PAHs). In naphthalene degradation test, 64 and 47% residual naphthalene was extracted after ten days of incubation from culture medium of?K. rosea?CMG2042 and?K. flava?CMG2028, respectively, when provided?with?0.5 mg ml-1?concentration as sole carbon source.?Due to addition of?0.5 mg ml-1?yeast extract as additional carbon source,?residual naphthalene extracted was 41 and 55% from?K. rosea?CMG2042 and?K.?flavaCMG2028, respectively. Both strains exhibited growth on 0.01 mg ml-1phenanthrene and fluoranthene in yeast extract added or omitted medium but onlyK. rosea?CMG2042 degraded 9% phenanthrene as a sole carbon source. Both strains had growth on minimal agar plates coated with Iranian light crude oil as sole carbon source and on agar medium added with yeast extract.
机译：从原油污染的海滩收集的不同样品经过富集，以分离能够在萘，菲和荧蒽上生长的细菌菌株。将WST-1 {4- [3-（4-碘苯基）-2-（4-硝基苯基）-2H-5-四唑啉] -1,3-苯二磺酸盐}呼吸还原为有色甲maz表明：分离的菌株CMG2028，通过16s rRNA鉴定为“ Kocuria flava”，在萘和菲上生长，而CMG2042，鉴定为“ Kocuria rosea”，在所有三种“多环芳烃”上生长。在萘降解试验中，在培养十天后从ΔK培养基中提取出64％和47％的残留萘。 Rosea？CMG2042和？K。 flava？CMG2028分别提供浓度为？0.5 mg ml-1？作为唯一碳源时。由于添加？0.5 mg ml-1？酵母提取物作为额外的碳源，残留的萘萃取量为41和55。 ％来自？ Rosea分别为CMG2042和K.flavaCMG2028。两种菌株均在添加或不添加培养基（仅K）的酵母提取物中的0.01 mg ml-1菲和荧蒽上均表现出生长。 Rosea？CMG2042作为唯一碳源降解了9％的菲。两种菌株均在涂有伊朗轻质原油作为唯一碳源的极少琼脂平板上和在添加酵母提取物的琼脂培养基上生长。

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