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首页> 外文期刊>African Journal of Biotechnology >Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues
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Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues

机译：前体对on子水Hydro愈伤组织产生类黄酮的影响

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摘要

Callus tissue of?Hydrocotyle bonariensis?was initiated from the leaf of?H. bonariensis?treated with 2 mg/l of 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). To optimize the precursors to increase the production of flavonoid, different precursors were used. The data showed that 4 mg/l proline produced the highest flavonoid yield (10.77 ± 0.25 mg/g DW). The increase in proline concentration did not significantly increase the production of flavonoid. The highest flavonoid yield (10.59 ± 0.18 mg/g DW) was produced in 1 mg/l of glutamine. No significant increase was attained in the flavonoid yield in callus treated with 2, 3 mg/l compared with the control. Phenylalanine at the concentration of 3 mg/l, successfully triggered the production of flavonoid (11.43 ± 0.12 mg/g DW), which was 23% higher than the control. The highest flavonoid production was attained in calluses treated with 4 mg/l of naringenin; and it was 19.72% higher compared with the control.
机译：co鱼的愈伤组织是从？的叶子开始的。用2 mg / l的2,4-二氯苯氧基乙酸（2,4-D）和1 mg / l的激动素处理bonariensis。将培养物在光照（冷白色荧光灯管，1200 lux）下保持在25℃。为了优化前体以增加类黄酮的产量，使用了不同的前体。数据显示4 mg / l的脯氨酸可产生最高的类黄酮产量（10.77±0.25 mg / g DW）。脯氨酸浓度的增加并未显着增加类黄酮的产量。 1 mg / l的谷氨酰胺可产生最高的类黄酮产量（10.59±0.18 mg / g DW）。与对照相比，用2、3 mg / l处理的愈伤组织中的类黄酮产量没有显着增加。浓度为3 mg / l的苯丙氨酸成功触发了类黄酮的产生（11.43±0.12 mg / g DW），比对照高23％。用4 mg / l柚皮苷处理的愈伤组织中黄酮类化合物的产量最高。与对照组相比提高了19.72％。

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