• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Subcelluar localization of orf126 of Bombyx mori nucleopolyhedrovirus
【24h】

Subcelluar localization of orf126 of Bombyx mori nucleopolyhedrovirus

机译：家蚕核多角体病毒orf126的亚细胞定位

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In order to explore the mechanism of?orf126?of?Bombyx mori?nucleopolyhedro virus, the subcellular localization of ORF126 was conducted. The?egfp?gene was fused with the C-terminal of?orf126?genes, BmN cells were transfected with different plasmid DNA and the superinfection were performed at 12 h post transfection. The fluorescence was examined by confocal laser scanning microscopy at different time point after transfection. The results show that EGFP protein was uniformly present throughout the cytoplasm and nucleus either in expression alone or superinfection, however, the fluorescence of EGFP linked to ORF126s were present barely in the cytoplasm.
机译：为了研究家蚕核糖多角体病毒orf126的机制，进行了ORF126的亚细胞定位。将“ egfp”基因与“ orf126”基因的C端融合，用不同的质粒DNA转染BmN细胞，并在转染后12小时进行超级感染。转染后在不同时间通过共聚焦激光扫描显微镜检查荧光。结果表明，无论是单独表达还是重复感染，EGFP蛋白都均匀地存在于整个细胞质和细胞核中，但是，与ORF126s连接的EGFP荧光几乎不存在于细胞质中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号