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首页> 外文期刊>African Journal of Biotechnology >Comparison of callus induction and somatic embryogenesis of some Iranian cottons (Gossypium Spp.) with Coker 312 and histology of somatic embryogenesis
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Comparison of callus induction and somatic embryogenesis of some Iranian cottons (Gossypium Spp.) with Coker 312 and histology of somatic embryogenesis

机译：伊朗一些棉花（Gossypium Spp。）与Coker 312的愈伤组织诱导和体细胞胚发生的比较以及体细胞胚发生的组织学

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Callus induction and somatic embryogenesis from hypocotyl explants of some Iranian cottons spp. (Hashem abad, Kerman, Termez and Sepid) were compared with Coker 312 through induction and formation of embryogenic calli on medium of Murashige and Skoog (MS) with Gamborg vitamins (B5) supplemented with the following compositions: MSB1?(0.5 mg/l zeatin), MSB2?(1 mg/l zeatin), MSB3?(0.5 mg/l 2,4-dichlorophenoxyacetic acid, 0.1 mg/l kinetin), MSB4?(1 mg/l 2,4-D, 0.5 mg/l kinetin, 0.5 mg/l zeatin) and MSB5?(2 mg/l α-naphtalene-3-acetic acid, 1 mg/l kinetin, 0.75 mg/l MgCl2).?The optimum medium for the proliferation of embryogenic calli was MS medium containing B5?vitamins, 1 mg/l 2,4-D, 0.5 mg/l kinetin and 0.5 mg/l zeatin and the optimum medium for the development of somatic embryos was MS medium (NH4NO3?was removed and KNO3?amount doubled) containing B5vitamins, 40 g/l sucrose and without hormone.?Media MSB1, MSB2?and MSB4?gave the highest percentage (100%) of calli induction in Coker 312 but the lowest induction (46.66%) was observed when Hashem abad explants were cultured in the MSB3?medium.?Embryogenesis percentage of Termez (2.22 to 24.40%), Hashem abad (1.85 to 9.73%) and Sepid (9.06 to 22.28%) genotypes were significantly lower than that of Coker 312 (66.66 to 94.33%). The Kerman genotype did not show embryogenesis. In the histological studies, the different development stages of the embryos (globular, heart, torpedo and cotyledonary) together with callus cells were showed.
机译：一些伊朗棉种下胚轴外植体的愈伤组织诱导和体细胞胚发生。 （Hashem abad，Kerman，Termez和Sepid）与Coker 312进行了比较，方法是在Murashige和Skoog（MS）含Gamborg维生素（B5）的培养基上诱导并形成胚性愈伤组织，补充了以下成分：MSB1？（0.5 mg / l）玉米素），MSB2？（1毫克/升玉米素），MSB3？（0.5毫克/升2,4-二氯苯氧基乙酸，0.1毫克/升激动素），MSB4？（1毫克/升2,4-D，0.5毫克/升l激动素，0.5 mg / l玉米素，2 mg / lα-萘-3-乙酸，1 mg / l激动素，0.75 mg / l MgCl2）和MSB5α。含有B5？维生素，1 mg / l 2,4-D，0.5 mg / l激动素和0.5 mg / l玉米素的MS培养基，MS培养基（去除NH4NO3？和KNO3？的量）是体细胞胚发育的最佳培养基。含B5维生素，40 g / l蔗糖且不含激素的双倍培养基）。培养基MSB1，MSB2和MSB4在Coker 312中的愈伤组织诱导率最高（100％），但在哈希姆（Hashem）贫瘠时观察到最低的诱导率（46.66％）。外植体在MSB中培养Termez（2.22至24.40％），Hashem abad（1.85至9.73％）和Sepid（9.06至22.28％）基因型的3中胚发生率显着低于Coker 312（66.66至94.33％）。克尔曼基因型未显示出胚胎发生。在组织学研究中，显示了胚的不同发育阶段（球形，心脏，鱼雷和子叶）以及愈伤组织细胞。

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