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首页> 外文期刊>African Journal of Biotechnology >NaCl effect on in vitro sugarcane bud emergency
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NaCl effect on in vitro sugarcane bud emergency

机译：氯化钠对体外甘蔗芽应急的影响

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It is important to determine precisely salt effects on bud germination. In this study, an?in vitro?procedure was used for studying sugarcane bud germination under NaCl salinity stress with different concentrations (0, 17, 34 and 68 mM) using five cultivars:?NCo310, CP70-321, CP65-357, CP66-346 and CP59-73. Germination percentage of control ranged from 75% for variety CP66-346 to 100% for variety CP65-357 after 8 days of culture. Salt stress reduced the rate of germination in all varieties.? At the end of the 8th day, salt stress decreased the percentage of final germination at all NaCl concentrations used and for all varieties except variety CP66-346 which showed a stimulation of germination at 34 mM NaCl. However, NaCl stress effects on bud germination of the five varieties used in this study were very low with an average reduction of 9.22; 5.88; 5.71 and 1.04 % in the presence of NaCl, respectively, for?NCo310, CP70-321, CP65-357 and CP59-73; a little increase (3.08 %) was observed for CP66-346. Thus, NaCl stress delayed buds germination and globally reduced the percentage of final germination. Varieties CP59-73 and CP66-346 appeared to be more salt tolerant at this stage than the three other varieties.
机译：准确确定盐对芽萌发的影响很重要。在这项研究中，使用“体外”程序研究了使用五个品种的不同浓度（0、17、34和68 mM）的NaCl盐度胁迫下的甘蔗芽萌发：NCo310，CP70-321，CP65-357，CP66 -346和CP59-73。培养8天后，对照的发芽百分比范围从品种CP66-346的75％到品种CP65-357的100％。盐胁迫降低了所有品种的发芽率。在第8天结束时，盐胁迫降低了所有使用的NaCl浓度以及所有品种（CP66-346除外）的最终发芽率，品种CP66-346在34 mM NaCl时表现出发芽刺激。但是，NaCl胁迫对本研究中使用的五个品种的芽萌发的影响非常低，平均降低了9.22;而对NaCl胁迫的影响却很小。 5.88; NCo310，CP70-321，CP65-357和CP59-73在NaCl存在下分别为5.71％和1.04％;观察到CP66-346略有增加（3.08％）。因此，NaCl胁迫延迟了芽的发芽，并总体上降低了最终发芽的百分比。在这个阶段，CP59-73和CP66-346品种比其他三个品种更耐盐。

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