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首页> 外文期刊>African Journal of Biotechnology >Provision of low cost media options for in vitro culture of Celosia sp.
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Provision of low cost media options for in vitro culture of Celosia sp.

机译：为鸡冠花的体外培养提供低成本的培养基选择。

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The composition of culture medium used for shoot regeneration has a great influence on cost of materials making of media. This study was conducted to investigate the effect?of?using four kinds of commercial starch or flour as alternative gelling agents and coconut water as an organic additive in the culture medium on the number of shoots produced for eight weeks in culture. Explants from stem segments of?Celosiasp were cultured on medium containing either half strength of Murashige and Skoog (MS) or without MS combined with 100 g/l of each gelling agents and 70 ml/l coconut water. The findings showed?that the?use of young coconut water and alternative gelling agents have response to?in vitro?shoots regeneration of stem segments ofCelosia?sp.?Higher?revenue?(12.33 ± 7.27)?of?shoots?derived fromstem?segments?results?from?the cultured?media containing100 g/l of potato starch, 70 ml/l coconut water, household sugar and half strength concentration of MS (? MS). Statistical analysis found no significant difference (P> 0.05) between culture media with different concentrations of MS with the number of shoots produced.?The substitution of alternative gelling agents, use of coconut water and quantities of MS powder can reduce costs to medium preparation.
机译：用于芽再生的培养基的组成对培养基材料的成本有很大的影响。进行这项研究的目的是研究在培养基中使用四种商品淀粉或面粉作为替代胶凝剂并使用椰子水作为有机添加剂对培养八周的芽数的影响。在含有Murashige和Skoog（MS）一半强度或不含MS的培养基中，将？Celosiasp茎节的外植体与100 g / l的每种胶凝剂和70 ml / l的椰子水混合培养。研究结果表明，使用幼椰子水和其他胶凝剂对鸡冠花茎段的再生有较高的反应。茎的茎段再生较高（12.33±7.27）。从含有100克/升的马铃薯淀粉，70毫升/升的椰子水，家用糖和半浓度的MS（？MS）的培养基中分离得到的蛋白质片段。统计分析发现，在不同浓度的MS培养基中，所产生芽的数量之间没有显着差异（P> 0.05）。替代胶凝剂的替代，使用椰子水和一定量的MS粉可以降低培养基制备的成本。

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