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Microsatellite association with seed protein content and flowering time in Nigerian cowpea cultivars

机译:尼日利亚cow豆品种微卫星与种子蛋白含量和开花时间的关联

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Cowpea microsatellites were evaluated for their potential to be used as markers associated with seed protein content and plant flowering time. Seeds with high and low protein content as well as plants with early and late flowering time were selected from 104 Nigerian cowpea accessions. Fifteen?(15)?microsatellite primer pairs?were tested for amplification of microsatellite sequences using PCR and seven pairs amplified a?single-locus. Sequence analysis showed a high level of diversity between the analysed genotypes. Although,?none of the microsatellites allowed clustering for protein content, one primer pair, VM68, detected a (AG)12-repeat microsatellite allele, which was present only in plants with late flowering. This microsatellite might possibly be useful as a marker associated with late-flowering time.
机译:评估pea豆微卫星的潜力,将其用作与种子蛋白含量和植物开花时间相关的标记。从104个尼日利亚cow豆种质中选择了蛋白质含量高和低的种子以及开花时间早和晚的植物。使用PCR测试了15对(15)个微卫星引物对的微卫星序列扩增,对了7对扩增的单基因座。序列分析显示了所分析的基因型之间的高度多样性。尽管没有一个微卫星允许蛋白质含量成簇,但是一对引物VM68检测到(AG)12重复的微卫星等位基因,该等位基因仅存在于开花较晚的植物中。该微卫星可能可用作与迟花时间相关的标记。

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