首页> 外文期刊>African Journal of Biotechnology >Determination of ploidy level by flow cytometry and autopolyploid induction in cocoyam (Xanthosoma sagittifolium)
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Determination of ploidy level by flow cytometry and autopolyploid induction in cocoyam (Xanthosoma sagittifolium)

机译:流式细胞仪和自体多倍体诱导测定可可粉中的倍性水平

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A procedure for chromosome doubling of the white cocoyam (Xanthosoma sagittifolium) type?was established by using colchicine and oryzalin treatments to?in vitro?plantlets. Flow cytometry was successfully used for analyzing ploidy levels within three cocoyam types and regenerated plants. Treating?in vitro?white cocoyam with?0.05% colchicine for 7 days produced tetraploids.?The oryzalin treatment (0.05% for 3 days) produced only mixoploids, but it has in turn, a lower rate of mortality. Therefore,?in white cocoyam,?oryzalin could constitute an alternative to the use of colchicine, whenever the selection and stabilization of polyploids induced by this reagent will be achieved.
机译:通过使用秋水仙碱和米扎林处理体外培养小植株,建立了白色可可粉(矢状黄单胞菌)型染色体加倍的程序。流式细胞仪已成功用于分析三种椰油型和再生植物中的倍性水平。用0.05%秋水仙碱处理体外白色可可碱7天可产生四倍体。谷杂霉素处理(0.05%持续3天)仅产生混合倍体,但死亡率较低。因此,在白色的椰油酰胺中,只要能够实现由该试剂诱导的多倍体的选择和稳定,氟尿嘧啶就可以替代秋水仙碱。

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