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首页> 外文期刊>African Journal of Biotechnology >Enhancing rooting consistency in Rosa damascena scions
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Enhancing rooting consistency in Rosa damascena scions

机译：增强罗莎（Rosa damascena）接穗的生根一致性

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This study was undertaken to determine the rooting efficiency of Damask rose shoot explants on Murashige and Skoog medium (MS) supplemented with 4 different concentrations of 3-indole butyric acid (IBA) in quarter, half and full mineral nutrient strengths. It was revealed that full strength MS medium even without IBA resulted in a high percentage of root formation after two months of culture despite the fact that the highest number of roots was observed in MS full strength and 2.0 mgL-1?IBA. Results also show that gradual acclimatization was essential for subsequent establishment of plantlets in natural condition. The highest percentage of plantlet survival was observed in potting media mixture containing; sand, soil, organic materials and vermiculite. Several experiments were carried out to stimulate?in vitro?rooting of Damask rose. Application of different media such as MS, 1/2 MS and 1/4 MS with different concentrations of 3-indole butyric acid (IBA) such as 1.0, 2.0 and 4.0 mgL-1and finally after analyzing and comparing all experiments results; it was observed that the most suitable rooting media for enhancing rooting efficiency in Damask rose are free full MS medium supplemented with 2.0 mgL-1?IBA.
机译：进行这项研究来确定锦缎玫瑰花芽外植体在补充了4种浓度的3-吲哚丁酸（IBA）的Murashige和Skoog培养基（MS）的四分之一，一半和全部矿物质营养强度中的生根效率。结果表明，即使在没有IBA的情况下，全强度MS培养基在培养两个月后仍会导致高比例的根形成，尽管在MS全强度和2.0 mgL-1？IBA中观察到了最多的根。结果还表明，逐渐适应环境对于随后在自然条件下建立小植株至关重要。在含有以下成分的盆栽培养基混合物中观察到最高的幼苗存活率。沙子，土壤，有机物和ver石。进行了一些实验，以刺激锦缎玫瑰的离体生根。最后，在对所有实验结果进行分析和比较之后，应用不同的介质（例如MS，1/2 MS和1/4 MS）以及不同浓度的3-吲哚丁酸（IBA）（例如1.0、2.0和4.0 mgL-1）。据观察，最能提高锦缎玫瑰生根效率的生根培养基是补充有2.0 mgL-1？IBA的游离全MS培养基。

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