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In vitro growth response of Artemisia annua seeds to different concentrations of plant growth regulators

机译:青蒿种子对不同浓度植物生长调节剂的体外生长响应

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Artemisia annua?is a crop popularly known for its importance in the production of anti-malarial and possibly antibacterial agents and natural pesticides.?A. annua anamed?is a hybrid which is able to grow well in hot weather and still retain its anti-malarial characteristics. Seeds of?annua?were cultured on?Murashige and Skoog?(MS)?full strength without plant growth regulators, and also on MS medium augmented with varied concentrations of kinetin (0.025 to 0.1 mg/L) and?benzyl amino purine (BA)(0.025 to 0.1 mg/L), while the auxin used, naphthalene acetic acid (NAA 0.01 mg/L) was constant for all treatments. At 16 weeks, data was collected and results were analyzed.?The mean shoot and root length?of plantlets regenerated from seeds?was optimum for elongation, on 0.1 mg/L kinetin combined with 0.01 mg/L NAA.?Single shoots formed on auxin free MS fortified with 0.025 mg/L BA, gave the highest mean number of nodes. Callus formation was evident at concentrations above 0.05 mg/L BA in combination with 0.01 mg/L NAA.
机译:蒿(Artemisia annua)是一种作物,因其在生产抗疟药和可能的抗菌剂以及天然农药中的重要性而广为人知。 Annua anamed?是一种杂种,能够在炎热天气下生长良好,并且仍然保留其抗疟疾特性。在没有植物生长调节剂的Murashige和Skoog®(MS)全强度培养基上,以及在各种浓度的激动素(0.025至0.1 mg / L)和苄基氨基嘌呤(BA)增强的MS培养基上培养番荔枝种子。 (0.025至0.1 mg / L),而所用的生长素,萘乙酸(NAA 0.01 mg / L)在所有处理中均保持不变。在16周时,收集数据并分析结果。从种子再生的幼苗的平均枝和根长最适合于0.1 mg / L激动素和0.01 mg / L NAA的延长。用0.025 mg / L BA强化的无生长素的MS,结节数最高。浓度高于0.05 mg / L BA与0.01 mg / L NAA时,明显形成愈伤组织。

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