• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Evaluation of antifungal activity from Bacillus strains against Rhizoctonia solani
【24h】

Evaluation of antifungal activity from Bacillus strains against Rhizoctonia solani

机译：芽孢杆菌菌株对茄枯萎病菌的抗真菌活性评价

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study,?30?bacterial strains isolated from marine biofilms were screened for their antifungal activity against?Rhizoctonia solani?by dual culture assay. Two bacterial strains,?Bacillus subtilis?and?Bacillus cereus,?showed a clear antagonism against?R. solani?on potato dextrose agar (PDA) medium. The antagonistic activity of?B. subtilisagainst this pathogen persisted after one month of co-culture. An?in vitro?antagonistic assay using potato tuber slices was set up allowing both the screening of bacteria for their biocontrol proprieties and for their rotting effect on plant tissues. Potato tuber slices treated with?B. cereus?showed a heavy tissues rotting, probably due to the secretion of several enzymes. Whereas,?B. subtilis?did not show any tissues rotting on treated potato tuber slices. In addition, the potato tuber slices treated with?B. subtilis?and subsequently inoculated with?R. solani?agar culture showed a reduced fungal infection in comparison to?the?control tuber slices inoculated with the pathogen only. The crude extract of?B. subtilis?strain culture in Luria-Bertani? (LB) medium at 48 h of incubation showed a high antifungal activity against?R. solani?growth and no cytotoxic effect on Brine shrimp larvae.
机译：在这项研究中，通过双重培养测定法筛选了从海洋生物膜分离的30种细菌菌株对茄枯萎菌的抗真菌活性。两种细菌菌株枯草芽孢杆菌和蜡状芽孢杆菌对R表现出明显的拮抗作用。马铃薯葡萄糖琼脂（PDA）培养基上的solani?。 βB的拮抗活性。枯草杆菌抵抗这种病原体共培养一个月后仍然存在。建立了使用马铃薯块茎切片的体外拮抗试验，可以筛选细菌的生物防治特性以及对植物组织的腐烂作用。用？B处理过的马铃薯块茎片。蜡状芽孢杆菌显示出沉重的组织腐烂，可能是由于几种酶的分泌。鉴于？枯草杆菌未在处理过的马铃薯块茎切片上显示出任何腐烂的组织。另外，用？B处理马铃薯块茎片。枯草芽孢杆菌，然后接种R。与仅接种病原体的对照块茎切片相比，茄尼琼脂培养物显示出减少的真菌感染。 B的粗提物。枯草吗？Luria-Bertani的菌株培养？孵育48小时的（LB）培养基对ΔR具有很高的抗真菌活性。茄尼生长，对盐水虾幼虫无细胞毒性作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号