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首页> 外文期刊>African Journal of Biotechnology >Fourier transform infrared (FTIR) spectroscopy for identification of Chlorella vulgaris Beijerinck 1890 and Scenedesmus obliquus (Turpin) Ktzing 1833
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Fourier transform infrared (FTIR) spectroscopy for identification of Chlorella vulgaris Beijerinck 1890 and Scenedesmus obliquus (Turpin) Ktzing 1833

机译：傅里叶变换红外（FTIR）光谱法用于鉴定小球藻贝耶林克1890年和斜生小球藻（Turpin）Ktzing 1833年

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摘要

Fourier transform infrared (FTIR) spectroscopy was used in this study to identify and determine spectral features of?Chlorella vulgaris?Beijerinck 1890 and?Scenedesmus obliquus?(Turpin) Kützing 1833. Two cultures were grown in a chemically-defined media under photoautotrophic culture conditions isolated from eutrophic freshwater lake in Ankara.?For FTIR analyses, a view from the transmission region between 4000 and 500 cm-1?on the microscope was chosen. All FTIR spectra showed a closely similar sequence of 11 distinct bands and were assigned a range of vibrationally active chemical groups, including residual water (–OH), lipid (–CH2), cellulose (–C=O), protein (amide), nucleic acid (>P=O) and starch (–C–O). The nonparametric (Spearman) correlations revealed a high level of correlations between certain bands.Comparison of band intensities (normalized to amide I) using Mann-Whitney test demonstrated major differences in relative band intensities, with bands 1, 3 and 5 to 11 showing significant differences between the two algal species at the 99% significant level. While bands 8, 9 and 10 showed the highest variations (CV>30%) inC. vulgaris, bands 1, 2, 3 and 7 with the highest variations (CV>30%) in?S. obliquus. The results show that FTIR technique has the potential to become applicable for the determination of single cell biomass composition from phytoplankton communities.
机译：在这项研究中，使用了傅里叶变换红外（FTIR）光谱技术来鉴定和确定寻常小球藻，Beijerinck 1890和Obcenquus obliquus（Turpin）Kützing1833的光谱特征。两种培养物在光养养条件下在化学成分确定的培养基中生长为了进行FTIR分析，选择了显微镜在透射区域4000至500 cm-1之间的视图。所有FTIR光谱均显示11个不同频段的序列非常相似，并分配了一系列振动活性化学基团，包括残留水（–OH），脂质（–CH2），纤维素（–C = O），蛋白质（酰胺），核酸（> P = O）和淀粉（–C–O）。非参数（Spearman）相关性揭示了某些谱带之间的高度相关性。使用Mann-Whitney检验进行的谱带强度比较（标准化为酰胺I）显示相对谱带强度存在重大差异，其中谱带1,3和5至11显示出显着差异两个藻种之间的差异在99％的显着水平上。频带8、9和10在C中显示出最高的变化（CV> 30％）。寻常的，带1、2、3和7，在？S中变化最大（CV> 30％）。斜。结果表明，FTIR技术有可能适用于确定浮游植物群落中单细胞生物量的组成。

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