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首页> 外文期刊>African Journal of Biotechnology >Development and characterization of microsatellite loci for Fenneropenaeus penicillatus Alcock
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Development and characterization of microsatellite loci for Fenneropenaeus penicillatus Alcock

机译：Fenneropenaeus penicillatus Alcock微卫星基因座的开发和表征

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Eight novel microsatellite loci from the genome of?Fenneropenaeus penicillatus?Alcock were developed using the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats (FIASCO).?Thirty (30)?wild individuals were used to analyze the polymorphism of these eight microsatellite markers. The results show that the number of alleles per locus and the polymorphism information content ranged?from 2 to 7 and from 0.2076 to 0.7484, respectively. The observed and expected heterozygosity were 0.1724 to 0.9130 and 0.1639 to 0.7314, respectively. These microsatellite primers will be used for further population genetic studies, constructing genetic linkage maps or locating quantitative trait locus (QTL) of?F. penicillatus?Alcock.
机译：通过快速扩增包含重复序列的片段长度多态性（FIASCO）的协议，开发了快速分离协议，从？Fenneropenaeus penicillatus？Alcock基因组中开发了八个新的微卫星基因座。使用三十（30）个野生个体分析了这些基因组的多态性。八个微卫星标记。结果表明，每个基因座的等位基因数目和多态信息含量分别为2至7和0.2076至0.7484。观察到的杂合度和预期杂合度分别为0.1724至0.9130和0.1639至0.7314。这些微卫星引物将用于进一步的群体遗传研究，构建遗传连锁图或定位？F的数量性状基因座（QTL）。 penicillatus？Alcock。

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