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首页> 外文期刊>African Journal of Biotechnology >Genetic variability of camel (Camelus dromedarius) populations in Saudi Arabia based on microsatellites analysis
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Genetic variability of camel (Camelus dromedarius) populations in Saudi Arabia based on microsatellites analysis

机译：基于微卫星分析的沙特阿拉伯骆驼（Camelus dromedarius）种群遗传变异

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The genetic diversity and population genetic structure of dromedary camels (Camelus dromedarius) are poorly documented in Saudi Arabia. The present study was conducted to address some of these genetics using four Saudi Arabian camel populations namely; Magaheem (MG), Maghateer (MJ), Sofr (SO) and Shual (SH). Genomic DNA was extracted from the hair roots of 160 camels, 40 individuals from each population. Sixteen microsatellite markers were used to genotype these 160 camels. Out of these 16 markers, only microsatellite?VOLP67?did not produce any polymerase chain reaction (PCR) amplicons. There were 139 alleles generated by the 15 microsatellites loci with a mean of 9.27 alleles per locus. Four of the microsatellites loci studied in MG, eight in MJ and six in both SO and SH were found to be deviated from Hardy-Weinberg equilibrium. The fixation genetic indices (Fst) among the four populations were very low, ranging from 0.006 (between SH and SO) to 0.017 (between MG and MJ), indicating low population differentiation among the four Saudi camel populations. No significant heterozygote excess or bottleneck in most nearest past was detected in the four camel populations as indicated by sign, standardized differences and Wilcoxon tests, along with the normal L shaped distribution of mode-shift test. The present study showed that the microsatellite markers are powerful tools in breeding programs, although there is a need for applying more microsatellites in order to be able to discriminate fairly between camel populations of Saudi Arabia.
机译：在沙特阿拉伯，单峰骆驼（Camelus dromedarius）的遗传多样性和种群遗传结构的文献很少。本研究是针对使用四个沙特阿拉伯骆驼种群的一些遗传学而进行的。 Magaheem（MG），Maghateer（MJ），Sofr（SO）和Shual（SH）。从160头骆驼的发根中提取基因组DNA，每个种群有40个个体。使用16个微卫星标记对这160个骆驼进行基因分型。在这16个标记中，只有微卫星VOLP67不会产生任何聚合酶链反应（PCR）扩增子。 15个微卫星基因座产生了139个等位基因，平均每个位点为9.27个等位基因。在MG中研究的四个微卫星基因座，在MJ中研究的八个，在SO和SH中研究的六个都被认为偏离了Hardy-Weinberg平衡。这四个种群之间的固定遗传指数（Fst）非常低，介于0.006（介于SH和SO之间）至0.017（介于MG和MJ之间），表明四个沙特骆驼种群之间的种群分化程度较低。如符号，标准化差异和Wilcoxon检验以及模式转换检验的正态L形分布所示，在四个骆驼种群中没有检测到最近的显着杂合子过量或瓶颈。本研究表明，微卫星标记是育种计划中的有力工具，尽管有必要应用更多的微卫星以能够公平地区分沙特阿拉伯的骆驼种群。

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