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Purification of an elicitor from Magnaporthe oryzae inducing defense resistance in rice

机译：稻瘟病菌诱导子在水稻中的抗性诱导

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Inducible defenses that contribute to overall resistance in plant can be triggered by elicitors. A novel elicitor, derived from the mycelia of the blast fungus?Magnaporthe oryzae, was purified to homogeneity by HiPrep 16/20 DEAE-Sepharose FF, Concanavalin A-Sepharose 4B and HiPrep 16/60 Sephacryl S-100 column chromatography.?The purified elicitor appeared as single band corresponding to a molecular weight of 48.53 kDa on?sodium dodecyl sulfate-polyacrylamide gel electrophresis (SDS-PAGE)?and?a pI?of 6.01 on isoeletric focusing (IEF) gel. Treatment with the purified elicitor increased the activities of?phenylalanine ammonium-lyase?(PAL)??and?peroxidase?(POD)?in rice susceptible cultivar CO39. Time-course analysis showed peak accumulation of PAL appeared at 24 h after treatment, and it was higher in challenge-inoculated plants than non-challenge plants. POD accumulation showed similar kinetics with PAL, but the largest peak appeared at 36 h after treatment. Compared to the untreated control plants, pretreatment of rice leaves with the purified elicitor provided an enhanced level of protection against?M. oryzae.?N-terminal blocked?elicitor?was identified as hypothetical protein MG 05155.4with 26.28% mass fingerprint coverage?by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry?(MALDI-TOF MS). The results suggest that the purified elicitor is involved in inducing resistance against blast fungus.
机译：引发子可以触发导致植物总体抗性的诱导防御。通过HiPrep 16/20 DEAE-Sepharose FF，伴刀豆球蛋白A-Sepharose 4B和HiPrep 16/60 Sephacryl S-100柱色谱法纯化了一种来自稻瘟病菌菌丝体稻瘟病菌菌丝的新型引发剂。激发子在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上显示为分子量为48.53 kDa的单条带，在等电聚焦（IEF）凝胶上显示pI为6.01。用纯化的激发子处理可提高水稻易感品种CO39的“苯丙氨酸铵裂合酶”（PAL）β和“过氧化物酶”（POD）β的活性。时程分析表明，在处理后24小时，PAL出现了峰值积累，并且在接种挑战的植物中PAL的积累高于未挑战植物。 POD积累显示出与PAL相似的动力学，但最大峰出现在处理后36小时。与未处理的对照植物相比，用纯化的激发子对稻叶进行预处理可提高对ΔM的保护水平。通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）将“ N末端封闭的激发子”鉴定为假设蛋白MG 05155.4，其指纹覆盖率为26.28％。结果表明，纯化的激发子参与诱导对稻瘟菌的抗性。

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