• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Inter-simple sequence repeat (ISSR) polymorphism-based analysis of diversity in the freshwater turtle genus Pangshura
【24h】

Inter-simple sequence repeat (ISSR) polymorphism-based analysis of diversity in the freshwater turtle genus Pangshura

机译：基于简单序列间重复（ISSR）多态性的淡水龟属庞氏菌的多样性分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Assessment of genetic variability among the freshwater turtles is critical to the development of effective conservation strategies. In the present study, analysis of phenetic relationship of the genus?Pangshura?was studied using?inter simple sequence repeat (ISSR) markers??to see the concordance of currently available morphological data with genetic data along with spatial pattern of distribution.?Ten microsatellite-based primers amplified 156 ISSR markers among 36 individuals belonging to four turtle species?Pangshura sylhetensis, Pangshura tecta, Pangshura simithii?and?Pangshura tentoria, collected from certain localities of Assam, West Bengal and Uttar Pradesh of India. Estimations of Nei’s genetic diversity (h), Shannon’s Index (I) and Total genetic diversity (Ht) reveal the existence of greater genetic diversity in?P. tentoria?and?P. sylhetensis?than in the other two species. However, low values of gene flow (Nm) and of within sample diversity (Hs) indicate prevalence of inbreeding in these species groups. The coefficients of differentiation (GST) values divulge that divergence started in the?Pangshura?species. Projections on the?principal components analysis (PCA)?plot reflect the distinct genetic identity of these four species. The consensus Neighbour Joining dendogram depicts?P. sylhetensis,?P. tecta, and?P. tentoria?as genetically closer to each other than to?P. smithii.
机译：评估淡水龟之间的遗传变异性对于制定有效的保护策略至关重要。在本研究中，使用“内部简单序列重复”（ISSR）标记研究了邦舒拉属的物候关系分析，以了解当前可用的形态学数据与遗传数据以及空间分布格局的一致性。基于微卫星的引物从印度阿萨姆邦，西孟加拉邦和北方邦的某些地区收集的36种个体中的156种ISSR标记物，这些个体属于四种turtle类，即印度庞古拉，庞古拉tecta，印度洋simithii和印度洋。 Nei的遗传多样性（h），Shannon指数（I）和总遗传多样性（Ht）的估算表明，？P中存在更大的遗传多样性。 Tentoria和P. sylhetensis？比其他两个物种都多。但是，基因流量（Nm）和样品多样性（Hs）内的值较低，表明这些物种组中近交的流行。分化系数（GST）值随着'Pangshura'物种的扩散而扩散。 “主成分分析”（PCA）图上的投影反映了这四个物种的独特遗传特性。共识邻居加入树状图描述了？西尔希特州tecta和？ Tentoria在遗传上彼此比在P附近更近。史密斯二世。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号