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首页> 外文期刊>African Journal of Biotechnology >Comparative genetic diversity studies in mustard (Brassica Juncea) varieties using randomly amplified polymorphic DNA (RAPD) analysis
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Comparative genetic diversity studies in mustard (Brassica Juncea) varieties using randomly amplified polymorphic DNA (RAPD) analysis

机译：芥菜（Brassica Juncea）品种的遗传多样性比较研究，使用随机扩增多态DNA（RAPD）分析

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Four random primers?was?utilized for detecting genetic diversity of five mustard varieties?as the detection of genetic diversity is essential for proper use in the genetic improvement of the mustard. A total of 20 bands were scored corresponding to an average of five bands per primer. Level of genetic polyphorphism was in the range of 0 to 66.66%. A dendrogram constructed using the unweighted pair group method with arithmetic averages (UPGMA) revealed the maximum similarity of variety Kanti with Varuna (similarity indices 0.9677) while distantly related varieties were Basanti and Rohini. The randomly amplified polymorphic DNA (RAPD) cluster pattern showed two major clusters namely cluster-I and cluster -II. Cluster -I includes Kanti and Varuna and cluster -II includes Rohini and Urvashi. The variety Basanti occupies a distinct place as revealed in the dendrogram.?Keywords:?Mustard, Genetic diversity,?RAPD,?UPGMA.
机译：四个随机引物被用于检测五个芥菜品种的遗传多样性，因为遗传多样性的检测对于芥菜遗传改良的正确使用至关重要。总共打了20条带，对应于每个引物平均5条带。基因多态性的水平在0至66.66％的范围内。使用具有算术平均值的非加权成对组方法构建的树状图显示，Kanti品种与Varuna的最大相似性（相似性指数为0.9677），而远缘相关的品种为Basanti和Rohini。随机扩增的多态性DNA（RAPD）簇模式显示了两个主要簇，即簇-I和簇-II。群集-I包括Kanti和Varuna，群集-II包括Rohini和Urvashi。如树状图所示，Basanti品种占有独特的位置。关键词：芥末，遗传多样性，RAPD，UPGMA。

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