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首页> 外文期刊>African Journal of Biotechnology >Micropropagation of Dioscorea alata L. from microtubers induced in vitro
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Micropropagation of Dioscorea alata L. from microtubers induced in vitro

机译：薯induced在体外诱导微块茎的微繁

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A new method made up of different steps was established for micropropagation ofDioscorea alata. First plantlets were regenerated from shoots proliferating on nodal cuttings cultured on half strength Murashige and Skoog salt medium (MS/2) (basal medium) supplemented with 0.5 mg l-1?BAP and 1 mg l-1?NAA. These plantlets were used to induce microtubers on the basal medium supplemented with 1 to 5 mg l-1?6-benzylaminopurine (BAP), kinetin (Kin) or α-naphthalene acetic acid (NAA) and 10 to 60 g l-1?sucrose. BAP at 2 to 3 mg l-1?combined with 20 to 30 g l-1?sucrose was more effective than Kin and NAA. It gave rise to 92% plantlets producing microtubers and the highest numbers of microtubers per plantlet varied between five and six. Microtubers, when sectioned and cultured on the basal medium supplemented with different BAP/NAA or Kin/NAA ratios, differentiated into shoots that, when isolated and subcultured in the same media, gave rise to rooted plantlets. The highest percentage of microtubers that differentiated into shoots was 98.8%, the highest number of shoots per microtuber was 7.5 and hence the highest number of rooted plantlets regenerated from those shoots was induced with BAP/NAA ratio (3/2 mg l-1)compared to that of BAP/NAA and Kin/NAA ratios. When the plantlets were acclimatized in different substrates, 97% survived in the mixture black soil/sand at equal volume (V/V) and this was the best result for the final step of the micropropagation of?D. alata?in this study. The different steps here described, allowed the regeneration of 45 and 16 plantlets from microtubers in about 251 days using BAP and NAA, respectively, and constituted a new and rapid method for the production of healthy seeds of this species.
机译：建立了由不同步骤组成的新方法，用于薯micro的微繁殖。从生长在半强度Murashige和Skoog盐培养基（MS / 2）（基础培养基）中补充了0.5 mg l-1？BAP和1 mg l-1？NAA的结节插条上繁殖的枝条再生出第一批小苗。这些小苗用于在补充了1至5 mg l-1？6-苄基氨基嘌呤（BAP），激动素（Kin）或α-萘乙酸（NAA）和10至60 g l-1？l的基础培养基上诱导微块茎。蔗糖。 2至3 mg l-1？的BAP与20至30 g l-1？蔗糖的结合比Kin和NAA更有效。它产生了92％的可生产微块茎的小植株，每株小植株的最大微块数量在5到6之间。将微型块茎切成薄片并在补充了不同BAP / NAA或Kin / NAA比例的基础培养基上培养后，会分化成芽，当在相同的培养基中分离和继代培养时，会产生生根的小植株。分化为芽的微块茎的最高百分比为98.8％，每个微块茎的最高芽数为7.5，因此，以BAP / NAA比（3/2 mg l-1）诱导从这些芽中再生的生根小植株的数量最高。与BAP / NAA和Kin / NAA的比率相比。当小苗在不同的基质上适应时，97％的黑土/沙土以相同的体积（V / V）存活，这对于ΔD微繁殖的最后一步是最好的结果。 alata？在这项研究中。此处描述的不同步骤分别允许使用BAP和NAA在约251天的时间内从微型块茎中再生出45株和16株小植株，并构成了一种新的快速方法来生产该物种的健康种子。

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