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首页> 外文期刊>African Journal of Biotechnology >Effect of media composition and explant type on the regeneration of eggplant (Solanum melongena L.)
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Effect of media composition and explant type on the regeneration of eggplant (Solanum melongena L.)

机译：培养基组成和外植体类型对茄子（Solanum melongena L.）再生的影响

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Two as well as three way interactions of three eggplant genotypes, media compositions and explants (hypocotyl, cotyledon and leaf) showed significant differences for plant regeneration. Among three explants, hypocotyl induced highest percent callusing, but cotyledon showed best results for somatic embryogenesis on all the media compositions. For three way interactions, cotyledon of BSR-27 induced significantly highest somatic embryogenesis (94.47%) on Murashige and Skooge (MS) fortified with 1.5 mgl-1?indole butyric acid (IBA) + 1.0 mgl-1?6-benzyl aminopurine (BAP). However, hypocotyl of BR-16 was not able to induce somatic embryogenesis on MS media fortified with 1.5 mgl-1?IBA + 1.0 mgl-1?BAP. Moreover, the embryogenic callus from cotyledon of BSR-27 (72.24%) achieved highest plant regeneration from somatic embryos on MS supplemented with 2.5 mgl-1?BAP + 1.0 mgl-1kin + 0.2% activated charcoal. Therefore, the performance of cotyledon explant of? BSR-27 is the best on MS fortified with 1.5 mgl-1?IBA + 1.0 mgl-1?BAP and 2.5 mgl-1?BAP + 1.0 mgl-1kin + 0.2% activated charcoal for somatic embryogenesis and plant regeneration, respectively.
机译：三种茄子基因型，培养基成分和外植体（下胚轴，子叶和叶片）的两种和三种相互作用都显示出植物再生的显着差异。在三个外植体中，下胚轴诱导了最高的愈伤组织百分数，但子叶在所有培养基成分上均显示出最佳的体细胞胚发生结果。对于三向相互作用，BSR-27的子叶在Murashige和Skooge（MS）强化1.5 mgl-1？吲哚丁酸（IBA）+ 1.0 mgl-1？6-6-苄基氨基嘌呤（ BAP）。但是，BR-16的胚轴不能在以1.5 mgl-1？IBA + 1.0 mgl-1？BAP强化的MS培养基上诱导体细胞胚发生。此外，BSR-27子叶的胚性愈伤组织（72.24％）在补充2.5 mgl-1BAP + 1.0 mglkin + 0.2％活性炭的MS上从体细胞胚获得了最高的植物再生率。因此，子叶外植体的表现如何？ BSR-27在强化了1.5 mgl-1？IBA + 1.0 mgl-1？BAP和2.5 mgl-1？BAP + 1.0 mgl-1kin + 0.2％活性炭的MS上是最好的，分别用于体细胞胚发生和植物再生。

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