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首页> 外文期刊>African Journal of Biotechnology >Effect of nodal positions, seasonal variations, shoot clump and growth regulators on micropropagation of commercially important bamboo, Bambusa nutans Wall. ex. Munro
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Effect of nodal positions, seasonal variations, shoot clump and growth regulators on micropropagation of commercially important bamboo, Bambusa nutans Wall. ex. Munro

机译：节点位置，季节变化，枝条和生长调节剂对商业上重要的竹子楠竹壁微繁殖的影响。例如门罗

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摘要

An efficient protocol for in vitro micropropagation of Bambusa nutans Wall. ex. Munro has been described. Nodal explants obtained from 1?-year-old field-grown culms of B. nutans produced up to 7.0 multiple shoots per explant on Murashige and Skoog (MS) basal medium supplemented with 6- benzylaminopurine (BAP, 1.0 mg/L). Continuous shoot proliferation up to 11.33 shoots was achieved by sub-culturing shoot clumps (4 shoots/cluster) in BAP (0.5 mg/L) and 0.1 mg/l α-naphthalene acetic acid (NAA) fortified medium every 4 weeks. 85% rooting was recorded on 2.0 mg/L NAA supplemented medium after 30 to 35 days of culture period. Micropropagated plantlets of B. nutans showed 70% survivability during the hardening stage. After hardening, rooted plantlets were successfully transferred to the soil and exhibited 80% survivability and normal growth. Plantlets cultivated in field condition achieved 95% survivability. Seed explants were also used for in vitro culture establishment of B. nutans on different combination of MS medium.
机译：南美斑潜蝇壁微繁殖的有效方案。例如已经描述了Munro。从1？岁田间生长的B. nutans茎获得的结点外植体在Murashige和Skoog（MS）基础培养基中补充了6-苄基氨基嘌呤（BAP，1.0 mg / L），每个植株最多产生7.0个新芽。每4周在BAP（0.5 mg / L）和0.1 mg / lα-萘乙酸（NAA）强化培养基中传代培养枝块（4枝/簇），可实现高达11.33枝的连续芽增殖。培养30至35天后，在2.0 mg / L NAA补充培养基上记录了85％的生根。在硬化阶段，B。nutans的微繁殖苗显示出70％的存活率。硬化后，生根的小苗成功地转移到土壤中，表现出80％的存活率和正常生长。在田间条件下种植的小植株存活率达95％。种子外植体也被用于在MS培养基的不同组合上体外培养B.nutans。

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