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Antioxidant activities in extracts of selected indigenous vegetables from Kenya and Malawi

机译:肯尼亚和马拉维精选本土蔬菜提取物中的抗氧化活性

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Antioxidant activities and phytochemical compounds of ethanol and hot water extracts of 7 selected indigenous vegetable species from Malawi and Kenya were Spectophotometrically determined and evaluated. Their effectiveness were also evaluated by their EC50 values through interpolation from linear regression analysis of their respective data. Generally, ethanolic extracts portrayed high quantities of total phenol, carotenoids and lycopene while hot water extracts showed high ascorbic acid. The highest total phenol (475.88±0.02 mg/g) and lycopene (0.13±0.02 mg/g) were detected in the ethanol extracts of I. batatas and C. gynandria, respectively. In the hot water extracts, the highest ascorbic acid (2.59±0.06 mg/g) and flavonoids (156.43±0.02 mg/g) were from M. esculenta. Dose-dependent antioxidant activities of the extracts were observed. Based on the EC50 values (mg/ml), the hot water extracts were significantly (p<0.05) more effective in all antioxidant activities assayed (DPPH, hydroxyl, superoxide anion radicals and reducing power) than ethanol extracts. It was observed that a single vegetable species did not posses all sorts of antioxidant phytochemical compounds in significant quantities and hence not effective in scavenging all different radicals. A combinatory intake of these vegetables species in sufficient concentrations should thus be recommended to enhance an optimal antioxidant capacity in the body.
机译:用分光光度法测定并评估了7种精选的马拉维和肯尼亚本土蔬菜的乙醇和热水提取物的抗氧化活性和植物化学成分。还通过对各自数据进行线性回归分析,通过内插法通过EC50值评估了其有效性。通常,乙醇提取物表现出大量的总酚,类胡萝卜素和番茄红素,而热水提取物表现出较高的抗坏血酸。在棉竹和and子的乙醇提取物中分别检测到最高的总酚(475.88±0.02 mg / g)和番茄红素(0.13±0.02 mg / g)。在热水提取物中,最高的抗坏血酸(2.59±0.06 mg / g)和类黄酮(156.43±0.02 mg / g)来自M. esculenta。观察到提取物的剂量依赖性抗氧化活性。根据EC50值(mg / ml),热水提取物在所有测定的抗氧化剂活性(DPPH,羟基,超氧阴离子自由基和还原能力)方面均比乙醇提取物有效(p <0.05)。观察到单一的植物物种没有大量的各种抗氧化剂植物化学化合物,因此不能有效地清除所有不同的自由基。因此,应建议摄入足够浓度的这些蔬菜,以增强体内的最佳抗氧化能力。

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