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首页> 外文期刊>African Journal of Biotechnology >Heritability of polyphenols, anthocyanins and antioxidant capacity of Cameroonian cocoa (Theobroma cacao L.) beans
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Heritability of polyphenols, anthocyanins and antioxidant capacity of Cameroonian cocoa (Theobroma cacao L.) beans

机译：喀麦隆可可豆的多酚，花色苷遗传力和抗氧化能力

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This study investigates the heritability of polyphenolic, anthocyanin and antioxidant capacity of beans derived from four cocoa clones and their offsprings. These compounds were analyzed at 280 nm (polyphenols) and 520 nm (anthocyanins) by reversed-phase high-performance liquid chromatography (RP-HPLC) method using a photodiode array detector (PDA). The antioxidant capacity of methanolic extracts prepared from cocoa beans was measured by the DPPH and ABTS assays. Samples displayed catechin and epicatechin as the two main polyphenols. Epicatechin, which represents 1 to 5% of defatted cocoa seed powder, was hundred-fold higher than catechin while cyanidin-3-arabinoside was three-fold higher than cyanidin-3-galactoside. The two main anthocyanins found in our samples represent about 0.05% of defatted cocoa seed powder. All these compounds were genotype-dependent. Unidentified substances called A, B and C were also found in cocoa seeds. Substance A is discussed as a derivative of caffeic acid and an ester-bound compound. Substances B and C are oligomers of proanthocyanidins. Antioxidant capacity of cocoa beans obtained by 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay was higher than those obtained by DPPH assay. According to correlation tests, flavanols contributed better in the antioxidant capacity than anthocyanin. No maternal effect was detected in the transmission of polyphenol compounds suggesting a nuclear heritability.
机译：这项研究调查了来自四个可可克隆及其后代的豆类的多酚，花色苷的遗传力和抗氧化能力。通过使用光电二极管阵列检测器（PDA）的反相高效液相色谱（RP-HPLC）方法在280 nm（多酚）和520 nm（花色素）上分析这些化合物。通过DPPH和ABTS分析测定了可可豆制备的甲醇提取物的抗氧化能力。样品显示儿茶素和表儿茶素是两种主要的多酚。表儿茶素占脱脂可可种子粉的1％至5％，比儿茶素高100倍，而花色素3-阿拉伯糖苷比花色素3-半乳糖苷高3倍。我们样品中发现的两种主要花色苷约占脱脂可可种子粉末的0.05％。所有这些化合物都是基因型依赖性的。可可种子中还发现了未被识别的物质A，B和C。讨论了物质A是咖啡酸和酯键化合物的衍生物。物质B和C是原花色素的低聚物。 2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸（ABTS）法测定的可可豆的抗氧化能力高于DPPH法测定的可可豆的抗氧化能力，根据相关性测试，黄烷醇的抗氧化能力优于花青素：在多酚化合物的传播中未检测到母体作用，表明其核遗传力。

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