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首页> 外文期刊>African Journal of Biotechnology >Assessing the genetic diversity of 48 groundnut (Arachis hypogaea L.) genotypes in the Guinea savanna agro-ecology of Ghana, using microsatellite-based markers
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Assessing the genetic diversity of 48 groundnut (Arachis hypogaea L.) genotypes in the Guinea savanna agro-ecology of Ghana, using microsatellite-based markers

机译：使用基于微卫星的标记评估加纳的几内亚热带稀树草原农业生态系统中48种花生（Arachis hypogaea L.）基因型的遗传多样性

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Groundnut (Arachis hypogaea L.) is the most important grain legume in Ghana. However, its production is constrained by a myriad of biotic and abiotic stresses which necessitate the development and use of superior varieties for increased yield. Germplasm characterisation both at the phenotypic and molecular level is important in all plant breeding programs. The aim of this study was to characterise selected advanced breeding groundnut lines with different phenotypic attributes at the molecular level using simple sequence repeats (SSR) markers in Ghana. A total of 53 SSR markers were screened and 25 were found to be polymorphic with an average polymorphic information content (PIC) value of 0.57. Of the 48 groundnut genotypes studied, 67% showed very close relationship (~100% similarity) with one or more genotypes among themselves. In fact, there were 14 instances where two to three genotypes within the same sub-cluster exhibited 100% similarity even though they displayed different phenotypic attributes. The remaining 33% of the groundnut genotypes were distant from each other and could therefore serve as effective parental material for future work. In this study, the SSR-based markers were found to be quite discriminatory in discerning variations between and among groundnut lines even where the level of variation was low. Microsatellite-based markers therefore represent a useful tool for dissecting genetic variations in cultivated crops, especially groundnut.
机译：花生（Arachis hypogaea L.）是加纳最重要的谷物豆类。然而，其生产受到无数的生物和非生物胁迫的限制，这需要开发和使用优良品种以提高产量。在所有植物育种计划中，在表型和分子水平上的种质表征都很重要。这项研究的目的是在加纳使用简单的序列重复（SSR）标记物在分子水平上表征具有不同表型属性的精选先进育种花生品系。总共筛选了53个SSR标记，发现25个是多态的，平均多态信息含量（PIC）值为0.57。在研究的48种花生基因型中，有67％与一种或多种基因型之间显示出非常紧密的联系（〜100％相似性）。实际上，在14个实例中，即使同一子集群中的2至3个基因型表现出不同的表型属性，它们也表现出100％的相似性。其余33％的花生基因型彼此远离，因此可以作为将来工作的有效育儿材料。在这项研究中，即使在变异水平较低的情况下，基于SSR的标记在区分花生品系之间和之间的变异时也具有很大的歧视性。因此，基于微卫星的标记代表着一种有用的工具，可用于剖析栽培作物（尤其是花生）中的遗传变异。

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