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Investigation of Argania spinosa L. (Skeels) polyphenols growing in arid and semi-arid conditions

机译:在干旱和半干旱条件下生长的Argania spinosa L.(Skeels)多酚的研究

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Argania spinosa L. Skeels, belonging to the Argania genus of the Sapotaceae family, is a species native to Morocco and Algeria. ?Due to its perfect adaptation to soil and climate, this tree plays an important ecological role in a constantly threatened encroached desert region. To understand the biological role of polyphenols in making the argan tree adapts to its natural habitat, we conducted a comparative study in two of the tree development stations: Tindouf located in South-western Algeria and Stidia located in Northwest Algeria. High performance liquid chromatography (HPLC) and Gas chromatography-mass spectrometry (GC-MS) used for the analysis of flavonoids led to the identification of six flavonols (two types of myricetin, rutin, hyperoside, quercetin, kaempferol) in the leaves of Tindouf argan tree, and two molecules (myricetin and quercetin) in the leaves of Stidia argan tree. Other molecules presented are few. The determination of flavonoids by spectrophotometry revealed the richness of Tindouf argan in these compounds (20%) compared to that of Stidia argan tree (8.7%). Histolocalisation of the flavonoids, tannins and anthraquinone in the leaves and stems of the tree was done using fluorescence microscope to understand the role of these molecules in the protection of this tree in its environment.
机译:属于剑兰科的Argania属的Argania spinosa L. Skeels是摩洛哥和阿尔及利亚的原生物种。由于对土壤和气候的完美适应,这种树在不断受到威胁的沙漠地区中起着重要的生态作用。为了了解多酚在使摩洛哥坚果树适应其自然栖息地方面的生物学作用,我们在两个树木开发站进行了比较研究:位于阿尔及利亚西南部的Tindouf和位于阿尔及利亚西北部的Stidia。高效液相色谱法(HPLC)和气相色谱质谱法(GC-MS)用于分析类黄酮,导致鉴定了廷杜夫叶片中的六种黄酮醇(两种类型的杨梅素,芦丁,高丝苷,槲皮素,山奈酚)摩洛哥坚果树,以及Stidia摩洛哥坚果树叶子中的两个分子(杨梅素和槲皮素)。提出的其他分子很少。通过分光光度法测定类黄酮表明,相比于针叶树针头树(8.7%),这些化合物中的豆蔻头含量高(20%)。使用荧光显微镜对树的叶子和茎中的类黄酮,单宁和蒽醌进行了组织定位,以了解这些分子在保护其环境中的作用。

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