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Morph-physiological responses to water deficit in parental genotypes of Medicago truncatula recombinant inbred lines

机译:苜蓿重组体自交系亲本基因型对水分亏缺的形态生理响应

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Medicago truncatula?is an omni-Mediterranean species grown as an annual forage legume. In addition to its small genome size and simple genetics,?M. truncatula?harbors several attributes which make it an attractive model legume. In this study, we investigated morphological and photosynthetic responses to water deficit in parental genotypes of M. truncatula recombinant inbred lines. Ten parental lines were cultivated under three water regimes (100% of field capacity (FC), 50% FC and 30% FC) and were harvested at flowering time and at the end of their lifecycle. Results from ANOVA showed that variability of measured parameters was explained by the effects of line, treatment and their interaction with treatment factor recorded the highest values. Out of the 27 traits, 14 were influenced by the line x treatment interaction. High to moderate broad-sense heritability (H2) were observed for most of the traits under control treatment and drought stress. Most of the correlations between measured traits were positive under the three water regimes. The flowering time was positively correlated with aerial and root growth rate. The tolerance of lines to water deficit seems to depend, in particular, on their ability to maintain higher photosynthetic activity. In 30% FC, principal component analysis clustered lines into two groups. The Jemalong A17 line was the least affected for most phenotypic parameters. Hence, all populations of recombinant inbred lines derived from crosses between Jemalong A17 and the remaining lines were useful for the identification of the genetic determinants for tolerance to water deficit in M. truncatula.
机译:紫花苜蓿(Medicago truncatula?)是一种全地中海型物种,以一年生的牧草豆科植物生长。除了其小的基因组大小和简单的遗传学,? truncatula?拥有多种特性,使其成为有吸引力的豆科植物典范。在这项研究中,我们调查了M. truncatula重组自交系亲本基因型对水分亏缺的形态和光合响应。在三种水分制度下(100%田间持水量(FC),50%FC和30%FC)种植了10个亲本系,并在开花期和生命周期结束时收获。 ANOVA的结果表明,测线参数的差异是由谱线,处理的影响及其与处理因子的相互作用所记录的最高值所解释的。在27个性状中,有14个受线x处理相互作用的影响。在对照处理和干旱胁迫下,大多数性状均观察到了高至中等的广义遗传力(H2)。在三种水分制度下,测量的性状之间的大多数相关性都是正的。开花时间与气生和根生长速率呈正相关。品系对水分缺乏的耐受性似乎尤其取决于它们保持较高光合作用活性的能力。在30%FC中,主成分分析将线分为两组。对于大多数表型参数,Jemalong A17品系受影响最小。因此,源自Jemalong A17与其余品系之间杂交的重组自交系的所有种群都可用于鉴定对M. truncatula水分缺乏的耐受性的遗传决定因素。

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