首页> 外文期刊>African Journal of Biotechnology >Effective method to control Vibrio mimicus infection in channel catfish Ictalurus punctatus
【24h】

Effective method to control Vibrio mimicus infection in channel catfish Ictalurus punctatus

机译:控制斑纹I鱼Ictalurus punctatus拟态弧菌感染的有效方法

获取原文
           

摘要

Recently, a skin ulcerative disease caused by Vibrio mimicus has led to heavy economic losses in catfish, including yellow catfish, southern catfish, and Zhengchuan catfish in China. Currently, there was no effective method of controlling the outbreak of this disease. In this study, the bacterial isolates were obtained from dying channel catfish and identified as V. mimicus, which consist of formalin-inactivated V. mimicus (antigen). After first immunization, four weeks later, fishes were exposed to V. mimicus and the immune response was analyzed: Fish survival, respiratory burst activity of blood leukocytes, serum agglutination titers, and lysozyme activity, every week (during four weeks). Survival was up 90%. Respiratory burst activity of blood leukocytes, serum agglutination titers, and lysozyme activity were determined at 1, 2, 3, and 4 weeks after primary immunization. Immunization of channel catfish protected hosts against V. mimicus infection with a survival percentage of more than 90%. Respiratory burst activity of blood leukocytes was not affected by vaccination. Serum agglutination titer and lysozyme activity were significantly increased after immunization, in comparison with un-vaccinated control fish. The obtained results indicated that vaccination is an effective method to control the outbreaks of V. mimicus through regulation of the humoral immune response.
机译:最近,由模仿弧菌引起的皮肤溃疡病已导致cat鱼(包括中国的黄cat鱼,南方cat鱼和正川cat鱼)的重大经济损失。当前,没有控制这种疾病暴发的有效方法。在这项研究中,细菌分离株是从垂死的channel鱼中获得的,被鉴定为拟南芥,由福尔马林灭活的拟南芥(抗原)组成。初次免疫后,四周后,将鱼类暴露于拟南芥中,并对免疫反应进行分析:每周(四周)鱼类存活率,血液白细胞的呼吸爆发活性,血清凝集滴度和溶菌酶活性。生存率提高了90%。在初次免疫后第1、2、3和4周确定血液白细胞的呼吸爆发活性,血清凝集滴度和溶菌酶活性。通道cat鱼的免疫保护了宿主免受拟南芥感染,存活率超过90%。疫苗接种不会影响血液白细胞的呼吸爆发活性。与未接种疫苗的对照鱼相比,免疫后的血清凝集滴度和溶菌酶活性显着提高。获得的结果表明,接种疫苗是通过调节体液免疫反应来控制拟南芥爆发的有效方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号