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首页> 外文期刊>African Journal of Biotechnology >Isolation and characterization of a lytic bacteriophage (VB_PAnP_PADP4) against MDR- Pseudomonas aeruginosa isolated from septic wound infections
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Isolation and characterization of a lytic bacteriophage (VB_PAnP_PADP4) against MDR- Pseudomonas aeruginosa isolated from septic wound infections

机译：针对感染性伤口感染中分离出的MDR-铜绿假单胞菌的溶菌噬菌体（VB_PAnP_PADP4）的分离和鉴定

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Resistance to multiple drugs by pathogens has become the sweltering problem for the treatment of bacterial infections. To overcome this problem of drug resistance, several diversified bacteriophages can be used as one of the alternatives and attractive approaches. The present study aimed to detect, isolate and partially characterize the bacteriophage used against MDR-Pseudomonas aeruginosa isolated from wound infections. A total number of 130 pus samples were collected and processed for the bacterial isolates from which a potential pathogen (P. aeruginosa) was identified and characterized using morphological, biochemical and molecular techniques (16S r-DNA technique). Among the Pseudomonas isolates, 26 multidrug-resistant strains were detected by Kirby-Bauer disc diffusion method. Bacteriophages (n=15) were collected against the MDR-P. aeruginosa and one of the efficient lytic phage (PADP4) was selected for this study. The transmission electron microscopic studies revealed that the selected phage PADP4 morphological features resemble the Podoviridae family. Phage PADP4 had the highest stability in a broad range of pH, temperatures, and high burst size (270 phages/ infected bacterial cells). Further, the potential phage, PADP4 was co-cultured with MDR-Pseudomonas aeruginosa and showed a significant growth reduction at 12 h of incubation and at multiplicity of infection of 1. Irrespective of the drug resistant bacteria, phage PA DP4 showed perfect lytic activity against the MDR- P. aeruginosa. It has been clarified that PADP4 could be a future promising agent for controlling MDR-P. aeruginosa.
机译：病原体对多种药物的耐药性已成为治疗细菌感染的闷热问题。为了克服这种耐药性的问题，几种多样的噬菌体可以用作替代方法和有吸引力的方法之一。本研究旨在检测，分离和部分表征针对从伤口感染中分离出的MDR-铜绿假单胞菌的噬菌体。收集并处理了总共130个脓液样本，用于分离细菌，使用形态学，生化和分子技术（16S r-DNA技术）从中鉴定出潜在的病原体（铜绿假单胞菌）并进行了表征。在假单胞菌分离物中，通过柯比-鲍尔圆盘扩散法检测到26种耐多药菌株。针对MDR-P收集噬菌体（n = 15）。铜绿假单胞菌和一种有效的裂解噬菌体（PADP4）被选为这项研究。透射电子显微镜研究表明，所选噬菌体PADP4的形态学特征类似于Podoviridae家族。噬菌体PADP4在广泛的pH值，温度和高爆破大小（270个噬菌体/感染细菌细胞）中具有最高的稳定性。此外，潜在的噬菌体PADP4与铜绿假单胞菌-铜绿假单胞菌共培养，在孵育12小时和感染复数为1时显示出明显的生长减少。无论耐药菌如何，噬菌体PA DP4均显示出对细菌的完美裂解活性。 MDR-铜绿假单胞菌。已经阐明，PADP4可能是控制MDR-P的未来有希望的试剂。铜绿。

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