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首页> 外文期刊>African Journal of Biotechnology >Capacity building for genetically modified organism (GMO) detection in West Africa: Identifying a circulating GMO maize variety in Mali
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Capacity building for genetically modified organism (GMO) detection in West Africa: Identifying a circulating GMO maize variety in Mali

机译：西非转基因生物（GMO）检测能力建设：在马里确定正在循环的转基因玉米品种

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摘要

DNA-based methodology employing quantitative polymerase chain reaction (qPCR) has been successfully used to examine the incidence of genetically modified (GM) maize in Mali. This study aims to ascertain whether screening elements could also be used to detect GM maize. Fourteen maize varieties and one unknown dark color seeded variety from Mali were tested. DNA was extracted from three seeds of each variety. Three screening elements were used for qPCR amplification, the 35s promoter of the Cauliflower mosaic virus (CaMV), the nopaline synthase (NOS terminator) from Agrobacterium tumefaciens and the 35s promoter from the Figwort mosaic virus (FMV). The 14 varieties were negative for P35s CaMV (forward) and T-NOS (reverse) markers. In contrast, the unknown dark color seeded variety was positive with 94 bp PCR product. While, no DNA fragments were amplified using the FMV as the screening element. These data were supported by Ct values in which the 14 varieties had values above 50; whereas, the unknown variety showed values of 24.5 for P-35s-CaMV and 30 for the T-NOS. The study demonstrates the ability in detecting GM maize using screening elements and the usefulness of our laboratory in training and reinforcing regional concern about GMO circulation.
机译：利用定量聚合酶链反应（qPCR）的基于DNA的方法已成功用于检查马里转基因（GM）玉米的发生率。这项研究旨在确定筛选元素是否也可以用于检测转基因玉米。对马里的14个玉米品种和一种未知的深色种子进行了测试。从每个品种的三个种子中提取DNA。三种筛选元件用于qPCR扩增：花椰菜花叶病毒（CaMV）的35s启动子，根癌农杆菌的胭脂碱合酶（NOS终止子）和菲格特花叶病毒（FMV）的35s启动子。这14个变种的P35s CaMV（正向）和T-NOS（反向）标记均为阴性。相反，未知的深色种子品种的94 bp PCR产物为阳性。同时，没有使用FMV作为筛选元件扩增DNA片段。这些数据得到了Ct值的支持，其中14个品种的Ct值高于50。而未知品种的P-35s-CaMV值为24.5，T-NOS值为30。这项研究证明了使用筛选元素检测转基因玉米的能力，以及我们实验室在培训和加强对转基因生物流通的区域关注方面的有用性。

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