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首页> 外文期刊>African Journal of Biotechnology >Molecular diagnosis of phytoplasma transmission from zygotic embryos to in vitro regenerated plants of coconut palm (Cocos nucifera L.)
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Molecular diagnosis of phytoplasma transmission from zygotic embryos to in vitro regenerated plants of coconut palm (Cocos nucifera L.)

机译：从合子胚到椰子树体外再生植株的植原体传播的分子诊断

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The aim of this study was to investigate the transmission of the lethal yellowing disease (LYD) of coconut tree caused by a phytoplasma from the zygotic embryo to the regenerated plantlet in vitro. From a total of 30 trees, 150 mature coconut nuts where harvested. These nuts were used to extract 150 zygotic embryos. From this package, 96 zygotic embryos were used to regenerate 96 young coconut seedlings in vitro and the 54 others were used to extract total DNA. From the stem of the 30 palms at the stage 1 of the LYD, phloem sample were also collected. From the regenerated in vitro-plantlets at 6 months age, leaf sample were collected. From the molecular diagnosis by PCR, 80% of the phloem samples carried the 16S rRNA gene of the phytoplasma responsible for LYD. All the zygotic embryos and in vitro-plantlets regenerated were healthy. So, coconut zygotic embryos can be used for the safe exchange of genetic material regarding lethal yellowing disease. The regenerated in vitro plantlet are free of disease.
机译：这项研究的目的是调查由植原体引起的椰子树致死性黄化病（LYD）从合子胚到体外再生植株的传播。从总共30棵树中收获了150个成熟的椰子坚果。这些坚果用于提取150个合子胚。从该包装中，将96个合子胚用于体外再生96个椰子幼树，其余54个用于提取总DNA。从LYD第1阶段的30只棕榈的茎中，还收集韧皮部样品。从6个月龄的再生的体外小植株中收集叶片样品。通过PCR的分子诊断，韧皮部样本中有80％携带了负责LYD的植物质体的16S rRNA基因。所有合子胚和再生的体外植株均健康。因此，椰子合子胚可用于安全交换致命黄变病的遗传物质。再生的体外苗无病。

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