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Enhanced production of intracellular dextran dextrinase from Gluconobacter oxydans using statistical experimental methods

机译:使用统计学实验方法提高氧化葡糖杆菌的细胞内葡聚糖糊精酶的产量

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Optimization of the fermentation medium for DDase production by?Gluconaobacter oxydans?M5 was carried out in the shake flasks using two kinds of statistical methods. Four variables, namely glucose, tryptone, yeast extract and sodium chloride, were found to influence DDase production significantly by the Plackett-Burman screening. A four-factor five-level central composite design (CCD) was chosen to explain the combined effects of the four medium constituents. The optimum medium consisted of glucose (17.670 g/L), maltobiose (30 g/L), tryptone (12.198 g/L), yeast extract (13.528 g/L), ammonium nitrate (15 g/L), copper sulfate (0.01 g/L), zinc sulfate?(0.01 g/L), and sodium chloride (0.009 g/L); the initial pH 6.0 was set prior to sterilization. The DDase yield obtained from optimized medium increased by 17-fold (0.238 U/mL) or so. Under these optimal conditions, the experimental values agreed with the predicted values, indicating that the chosen method of optimization of medium composition was efficient, relatively simple, time reducing and material saving.
机译:利用两种统计方法,在摇瓶中优化了氧化葡糖杆菌M5产生DDase的发酵培养基。通过Plackett-Burman筛选发现,葡萄糖,胰蛋白,、酵母提取物和氯化钠这四个变量显着影响DDase的产生。选择了四因素五级中央复合设计(CCD)来解释四种培养基成分的综合作用。最佳培养基包括葡萄糖(17.670 g / L),麦芽二糖(30 g / L),胰蛋白((12.198 g / L),酵母提取物(13.528 g / L),硝酸铵(15 g / L),硫酸铜( 0.01g / L),硫酸锌(0.01g / L)和氯化钠(0.009g / L)。灭菌前设定初始pH 6.0。从优化培养基获得的DDase产量增加了17倍(0.238 U / mL)。在这些最佳条件下,实验值与预测值一致,表明所选择的培养基组成优化方法是高效,相对简单,省时和省料的。

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